Fig 1: Components of cell death signaling pathways are affected by AC100861.1 and TNFRSF10A dysregulation.Graphs indicate the expression level changes (compared to control) in ARPE-19 cells transfected with siRNA targeting AC100861.1 (A), AC100861.1 overexpression vector (B), siRNA targeting TNFRSF10A (C), and TNFRSF10A overexpression vector (D). Expression levels of TRAIL, CASP3, CASP8, FADD, and MLKL were measured by RT-qPCR. The gray dotted line corresponds to fold change = 1. For all samples, N = 3. Error bars indicate the standard error of the mean.
Fig 2: Phosphorylated MLKL levels change in response to AC100861.1 and TNFRSF10A dysregulation.Graph displaying the fold change of phosphorylated MLKL (phos-MLKL) protein levels in the AC100861.1-KD, AC100861.1-OE, TNFRSF10A-KD, and TNFRSF10A-OE samples compared to control samples. The gray dotted line corresponds to fold change = 1. For all samples, N = 3. Error bars indicate the standard error of the mean.
Supplier Page from Abcam for Phospho-MLKL (S345) and Total MLKL ELISA Kit