Fig 2: Association of ZC3H13 expression with m6A modification and immune infiltration in ESCC. (A, B) Colorimetric assay was used to measure intracellular m6a methylation. (C, D) Correlation between cellular ZC3H13 expression and m6a methylation levels. (E) Colorimetric method was used to detect m6a methylation in tissues. (F) IF staining was used to detect intracellular ZC3H13, WTAP, METTL14, and METTL3 (400×). (G–J) The relative expression of TNF-α, CD80, CD68 and CD206 in the ZC3H13-shrNA-NC (n = 5) and ZC3H13-shRNA (n = 5) groups was analyzed by qRT-PCR. (K, L) Representative images of CD68 & CD206 IHC staining in ZC3H13-shrNA-NC and ZC3H13-shRNA tumor tissues (200×). ns: not significant, *: p < 0.05, **: p < 0.01.