Fig 1: Macrophage RNF150 deficiency alleviates adipose tissue inflammation. (A) Representative immunohistochemical staining of TNF-α, IL-1β, IFN-γ, and F4/80 in epiWAT from HFD-fed RNF150fl/fl and RNF150ΔLysm mice. Magnification ×400, scale bar = 50 μm. (B) Quantification of positive staining areas from 5 random fields per section (n = 10 mice per group). (C) ELISA quantification of anti-inflammatory markers (CD163, CHIL3, ARG1, KLF4, and MERTK) in epiWAT (n = 10 per group). (D) ELISA quantification of proinflammatory markers (TNF-α, IL-1β, MCP1, IFN-γ, ADGRE1) in epiWAT (n = 10 per group). The data are presented as the means ± SDs. Statistical analysis was performed via Student's t-test. ∗∗P < .01.