Fig 1: Protein analysis of PTEN, Sema3B, TNF-α and TNFSF10 in the DMEM(LG) and DMEM(HG) groups. In passage 4 cells, the levels of PTEN, Sema3B, TNF-α and TNFSF10 were not significantly different between the DMEM(LG) group and the DMEM(HG) group. Relative expression levels were calculated as the relative change from the DMEM(HG) group. The test was performed on the cells from all the six donors. The experiment was performed in triplicate, and the mean value of every sample was used for group comparisons. Group comparisons were performed using the paired t-test. n = 6.