Mouse TNF-alpha Quantikine ELISA Kit MTA00B-1 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse TNF-alpha Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberMTA00B-1
Quantity1 Kit (for 1 Plate) (also 1 Pack (50 Plates), 1 Pack (6 Plates))
ApplicationsELISA
Detection Range10.90 - 700 pg/mL
ReactivityMouse
Sample TypeCell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity7.21 pg/mL
Detection TargetTNF-alpha
Assay Time4.5 hours
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesDIF, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tnfa, Tnfsf1a, Tnlg1f

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Targeted delivery of RNA using synthetic bacterial sporesJournal of Drug Delivery Science and TechnologyApril 27, 2026Federico Machinandiarena, Domenico D'Atri, Lisa M. Jenkins, Kandice Tanner, David J. Fitzgerald, Kumaran S. Ramamurthicollected and analyzed for TNF-α and IL-6 by sandwich ELISA using commercial kits (R&D Systems Cat. No. MTA00B-1 and M6000b-1, respectively). Absorbance was read at 450 nm with 570 nm as a reference wavelength, and

(2) Development of highly potent EP2 antagonists as novel anti-inflammatory neuroprotectantsBioorganic & Medicinal ChemistryMarch 24, 2026Chenyao Jiang, Eun Bee Cho, Zhen Jin, Sina Bahraminejad, Dejian Ma, Ying Yu, Jiawang Liu, Jianxiong Jiang#SMLB00C), IL-6 (R&D Systems, Cat. #M6000B-1), and tumor necrosis factor-α (TNF-α) (R&D Systems, Cat. #MTA00B-1) released into the culture medium were quantified using ELISA kits, according to the manufacturers’

(3) Lipid self-assembling nanoparticles as a novel platform for mRNA-based vaccinationMolecular Therapy Nucleic AcidsMarch 12, 2026Arianna De Chiara, Valeria Nele, Alessia Angelillo, Virginia Campani, Andrea Campanile, Guendalina Froechlich, Annagiulia Scognamiglio, Emilio Pellino, Antonietta Greco, Clizia Chinello, et al.sera using ELISA kits (IL-6 product reference: M6000B-1, R&D Systems Inc; TNF-α product reference: MTA00B-1, R&D Systems Inc.), according to the manufacturer’s instructions. Biodistribution experiments were performed

(4) Sorting nexin 5 mediates antigen presentation and immunity against Mycobacterium tuberculosisbioRxivFebruary 2, 2026Beatriz R. S. Dias, Kubra F. Naqvi, Victoria A. Ektnitphong, Samuel Alvarez-Arguedas, Kathryn C. Rahlwes, Priscila C. Campos, Michael U. Shilohremaining homogenate was centrifuged and filtered. IFN-γ (Invitrogen, 88–7314-88), TNF-α (R&D Systems, MTA00B-1), IL12p40 (R&D Systems, MP400), and IL-2 (Invitrogen, 88–7024-88) were measured by ELISA according to

(5) Magnolol Ameliorates Cisplatin-Induced Acute Kidney Injury with Activation of Nrf2-Associated Antioxidant ResponsesCurrent Issues in Molecular BiologyJanuary 17, 2026Mi-Gyeong Gwon, Min Hui Park, Jaechan Leemwere quantified by enzyme-linked immunosorbent assay kits (R&D Systems, Minneapolis, MN, USA; Cat# MTA00B-1, M6000B-1, and MLB00C-1). To evaluate oxidative stress, lipid peroxidation was quantified by measuring

(6) Pulmonary toxicity of polymethyl methacrylate nanoplastics via intratracheal intubation in miceScientific ReportsJanuary 6, 2026Changsic Youn, Yu-Jin Jo, Jeongwoo Kwon, Seung-Bin Yoon, Hyeong-Ju You, Ji-Su Kimlevels (IL-6, IL-1β, TNF-α), serum and BALF samples were tested using ELISA kits (M6000B-1, MLB00C-1, MTA00B-1, R&D Systems, Minneapolis, USA) according to the manufacturer’s instructions. BCA and ELISA signals

(7) Interleukin-38 is a negative regulator of trained immunity—A retrospective multi-omics studyiScienceNovember 21, 2025Lisa U. Teufel, Vasiliki Matzaraki, Lukas Folkman, Dennis M. de Graaf, Rob ter Horst, Simone J.C.F.M. Moorlag, Jéssica C. dos Santos, Catharina M. Mulders-Manders, Thomas Krausgruber, Charles Dinarello, et al.assays,Critical commercial assays,Critical commercial assays ,, Human/Murine TNF ELISA,R&D Systems,Cat#DY210/MTA00B-1 Human/Murine IL-6 ELISA,R&D Systems,Cat#DY206/DY406-05 Human IL-38 ELISA,R&D Systems,Cat#DY9110-05 Human/Murine

(8) Small Intestine Neuromuscular Dysfunctions and Neurogliopathy in a Mouse Model of High-Fat Diet-Induced Obesity: Involvement of Toll-Like Receptor 4International Journal of Molecular SciencesNovember 3, 2025Sofia Faggin, Silvia Cerantola, Annalisa Bosi, Cristina Giaroni, Eleonora Napoli, Edoardo Vincenzo Savarino, Martina Contran, Andrea Porzionato, Carolina Pellegrini, Luca Antonioli, et al.IL-6, and IL-1β were measured with specific ELISA kits (Mouse TNF-alpha ELISA Kit–Quantikine, cat# MTA00B-1; Mouse IL-6 Quantikine ELISA Kit, cat# M6000B-1; Mouse IL-1 beta/IL-1F2 ELISA Kit–Quantikine Kit, cat#

(9) Reprogramming of cancer metabolism via photoresponsive nano-PROTAC enhances pyroptosis-mediated immunotherapySignal Transduction and Targeted TherapySeptember 26, 2025Byeongmin Park, Jiwoong Choi, Jae-Hyeon Lee, Yelee Kim, Woohyeong Lee, Ansoo Lee, In-Cheol Sun, Hong Yeol Yoon, Yongju Kim, Sun Hwa Kim, et al.No. 116013, Biolegend; CD25+, Cat. No. 101904, Biolegend). In the coculture medium, TNF-α (Cat. No. MTA00b-1, R&D Systems), IFN-γ (Cat. No. MIF00-1, R&D Systems), and IL-10 (Cat. No. M1000B-1, R&D Systems) were

(10) cGAS-STING-NFκB PATHWAY PLAYS A ROLE IN BURN INJURY-INDUCED MUSCLE WASTINGShockSeptember 1, 2025Fei Xie, Zerong You, Bin Yan, Jiajia Dai, Jinsheng Yang, Shiyu Wang, Hiroki Ogata, Shingo Yasuhara, JA Jeevendra Martynshown in Table 1. The enzyme-linked immunosorbent assay for cytokines The mouse TNF-α ELISA Kit (MTA00B-1, R&D systems), IL-10 ELISA Kit (M1000B-1, R&D systems), and IL-6 ELISA Kit (M6000B-1, R&D systems) were

(11) Polystyrene particles induces asthma-like Th2-mediated lung injury through IL-33 secretionEnvironment internationalSeptember 1, 2025Woo JH, Song MK, Baek SH, Park CJ, Kim JW, Kim B, Lee K.

(12) Haematopoietic stem cell number is not solely defined by niche availabilityNatureAugust 27, 2025Shoichiro Takeishi, Tony Marchand, Wade R. Koba, Daniel K. Borger, Chunliang Xu, Chandan Guha, Aviv Bergman, Paul S. Frenette, Kira Gritsman, Ulrich Steidlmeasured using mouse IL-1β (BMS6002), IL-6 (KMC0061) ELISA kits (Thermo Fisher Scientific) and TNF (MTA00B-1), CXCL12/SDF-1α (MCX120), SCF (MCK00) and TPO (MTP00) Quantikine ELISA kits (R&D Systems) according

(13) RNA-seq analysis of shrimp tropomyosin-induced allergic reactions through PI3K/Akt pathwayFront NutrJuly 9, 2025Yanchu Li, Yuying Yang, Junyao Li, Rui Guo, Zhiqiang Niu, Weicheng Hu, Shucheng Liu, Shuai WeiTNF-α in the supernatant of RBL-2H3 cells were detected using commercial ELISA kits (D8000C; E-EL-0032; MTA00B-1; R&D Systems), following the manufacturer’s instructions. 2.10 Real-time qRT-PCR RNA was extracted

(14) Bone morphogenetic protein 10 serves as a biomarker and a potential therapeutic target for endothelial dysfunction in endotoxin-induced acute lung injuryJournal of translational medicineJuly 8, 2025Yu WK, Chen WC, Shen HC, Sun CY, Yu HJ, Su VY, Yang KY.

(15) Dendritic cell membrane-based DCsLipo@MnO2@siCTLA4@PD-1α nanomedicine for the treatment of Lynch syndrome-related colorectal cancerMater Today BioJuly 1, 2025Wenjin Zhong, Pengcheng Wang, Jintian Wang, Yuejia Zhu, Liquan Chen, Yilong Qian, Xiang Huang, Kai Yelevels of Granzyme B (NBP3-11787), Interferon-γ (IFN-γ) (MIF00-1), and Tumor Necrosis Factor-α (TNF-α) (MTA00B-1) in the co-culture system were measured with ELISA kits (Biotechne, USA). Specifically, we took 50 μL

(16) Complement system is activated in acute inflammatory response to environmental particulates in the lungsEcotoxicology and environmental safetyJune 1, 2025Zhang K, Honda A, Sagawa T, Miyasaka N, Qiu B, Ishikawa R, Okuda T, Kameda T, Sadakane K, Ichinose T, et al.

(17) Atractylenolide I Inhibits Nicotine-Induced Macrophage Pyroptosis and Alleviates Atherogenesis by Suppressing the TLR4/ROS/TXNIP/NLRP3 PathwayMetabolitesMay 15, 2025Li HH, Liu X, Wang YP, Xu X, Zhu L, Zhang W, Ren K.

(18) B cell–derived acetylcholine mitigates skin inflammation in mice through α9 nicotinic acetylcholine receptor–mediated signalingProceedings of the National Academy of SciencesApril 29, 2025Erica Foffi, Francesco Rugolo, Nisha Ramamurthy, Jillian Haight, Simone Helke, Annick You-Ten, Chantal Tobin, Soode Moghadas Jafari, Andrew J. Elia, Thorsten Berger, et al.quantifications of serum IL-6 and TNFα were performed using the appropriate kits (Cat. No. M6000B-1, MTA00B-1, R&D Systems) according to the manufacturer’s instructions. Briefly, whole blood was collected by cardiac

(19) Chaperone-mediated autophagy sustains pericyte stemness necessary for brain tissue homeostasisJournal of advanced researchApril 24, 2025Salinas MD, Martínez CM, Roca FJ, García-Bernal D, Martínez-Morga M, Rodríguez-Madoz JR, Prósper F, Zapata AG, Moraleda JM, Martínez S, et al.

(20) Tumor-derived arachidonic acid reprograms neutrophils to promote immune suppression and therapy resistance in triple-negative breast cancerImmunityApril 8, 2025Liqun Yu, Keziah Liebenberg, Yichao Shen, Fengshuo Liu, Zhan Xu, Xiaoxin Hao, Ling Wu, Weijie Zhang, Hilda L. Chan, Bo Wei, et al.Mouse IFN gamma ProQuantum Immunoassay Kit,Thermo Fisher Scientific,Cat# A41150 Mouse TNF-alpha ELISA Kit - Quantikine,R&D System,Cat# MTA00B-1 Deposited data,Deposited data,Deposited data Raw data of scRNA-seq of tumor infiltrate immune cells,This

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Brain, behavior, and immunity

Molecular Therapy Nucleic Acids

Mouse TNF-alpha Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Mouse TNF-alpha Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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