Fig 1: Effects of BMPR2 on the proliferation, migration and mortality of PMECs. A Expression levels of BMPR2 in hypoxia-treated PMECs (n = 4). B Expression levels of BMPR2 in PMECs treated with siRNA BMPR2 (n = 3). C–F Cell proliferation analysis, wound healing analysis, cell migration analysis and cell mortality analysis of PMECs transfected with siRNA BMPR2 under normoxia (n = 5 or 4). G–J Cell proliferation analysis, wound healing analysis, cell migration analysis and cell mortality analysis of PMECs cotransfected with miR-27a-3p inhibitor and siRNA BMPR2 under normoxia (n = 5 or 4). All data are presented as the mean ± SEM. Scale bar: 100 µm
Fig 2: BMPR2 is the target of miR-27a-3p. A Volcano map analysis of RNA sequencing in PAECs overexpressing circGSAP. B miRWalk, StarBase, and TargetScan bioinformatics software predicted mRNAs that could bind to miR-27a-3p and the intersection with RNA sequencing results. C qRT–PCR was used to detect mRNA that could bind to miR-27a-3p in PMECs treated with circGSAP (n = 3). D Expression levels of BMPR2 in PMECs treated with circGSAP siRNA (n = 3). E, F Expression levels of BMPR2 in PMECs treated with miR-27a-3p mimics and inhibitor (n = 3). G Expression levels of BMPR2 in lung tissues of IPAH patients (n = 4). H Expression levels of BMPR2 in lung tissues of control, MCT, MCT-AAV-NC and MCT-AAV group rats (n = 11). I, J The protein levels of BMPR2 in circGSAP-treated and siRNA circGSAP-treated PMECs (n = 4). K Dual-luciferase assays were used to validate the interactions between miR-27a-3p and BMPR2 (n = 3). All data are presented as the mean ± SEM. Scale bar: 100 µm
Fig 3: A schematic diagram illustrating the hypothetical model by which circGSAP adsorbed miR-27a-3p via a sponging mechanism and increased the BMPR2 signaling pathway, improving the overproliferation and migration, increasing mortality of PMECs in the remodeled pulmonary arteries
Supplier Page from Wuhan Fine Biotech Co., Ltd. for Human BMPR2(Bone morphogenetic protein receptor type-2) ELISA Kit