Fig 1: Expression profile of selected genes across VAS pain scores Bar graphs showing fold-change values (mean ± SD) of selected genes measured by RT-qPCR across VAS categories. Positive values indicate upregulation and negative values indicate downregulation in degenerated IVD in comparison to control samples.BCL2: B-cell lymphoma 2; C: control; CAT: catalase; CAV3: caveolin-3; CRP: C-reactive protein; CXCL8: C-X-C motif chemokine ligand 8 (interleukin-8); CYBA: cytochrome b-245 α chain (p22phox); G2–G5: grade of disc degeneration according to Pfirrmann’s scale; GPX1: glutathione peroxidase 1; ICAM1: intercellular adhesion molecule 1; IFNG: interferon γ; IL1B: interleukin 1 β; IL6: interleukin 6; JUN: Jun proto-oncogene (AP-1 transcription factor subunit); MAPK1: mitogen-activated protein kinase 1 (ERK2); MAPK10: mitogen-activated protein kinase 10 (JNK3); MAPK8: mitogen-activated protein kinase 8 (JNK1); MMP9: matrix metallopeptidase 9 (gelatinase B); NOS1: nitric oxide synthase 1 (neuronal NOS); NOS2: nitric oxide synthase 2 (inducible NOS); NOS3: nitric oxide synthase 3 (endothelial NOS); NRF1: nuclear respiratory factor 1; SIRT3: sirtuin 3 (NAD-dependent deacetylase); SLC2A1: solute carrier family 2 member 1 (glucose transporter 1, GLUT1); SOD1: superoxide dismutase 1 (Cu/Zn SOD); SOD2: superoxide dismutase 2 (Mn SOD, mitochondrial); SOD3: superoxide dismutase 3 (extracellular SOD); TLR4: toll-like receptor 4; TNF: tumor necrosis factor α; TP53: tumor protein p53.
Fig 2: Oxidative stress-related gene expression analyzed by RT-qPCR across Pfirrmann grades Bar graphs showing relative expression levels (fold change, mean ± SD) of selected genes measured by RT-qPCR. Positive values indicate upregulation and negative values indicate downregulation in degenerated IVD tissue compared with control samples.BCL2: B-cell lymphoma 2; C: control; CAT: catalase; CAV3: caveolin-3; CRP: C-reactive protein; CXCL8: C-X-C motif chemokine ligand 8 (interleukin-8); CYBA: cytochrome b-245 α chain (p22phox); G2–G5: grade of disc degeneration according to Pfirrmann’s scale; GPX1: glutathione peroxidase 1; ICAM1: intercellular adhesion molecule 1; IFNG: interferon γ; IL1B: interleukin 1 β; IL6: interleukin 6; JUN: Jun proto-oncogene (AP-1 transcription factor subunit); MAPK1: mitogen-activated protein kinase 1 (ERK2); MAPK10: mitogen-activated protein kinase 10 (JNK3); MAPK8: mitogen-activated protein kinase 8 (JNK1); MMP9: matrix metallopeptidase 9 (gelatinase B); NOS1: nitric oxide synthase 1 (neuronal NOS); NOS2: nitric oxide synthase 2 (inducible NOS); NOS3: nitric oxide synthase 3 (endothelial NOS); NRF1: nuclear respiratory factor 1; SIRT3: sirtuin 3 (NAD-dependent deacetylase); SLC2A1: solute carrier family 2 member 1 (glucose transporter 1, GLUT1); SOD1: superoxide dismutase 1 (Cu/Zn SOD); SOD2: superoxide dismutase 2 (Mn SOD, mitochondrial); SOD3: superoxide dismutase 3 (extracellular SOD); TLR4: toll-like receptor 4; TNF: tumor necrosis factor α; TP53: tumor protein p53.
Supplier Page from Fine Biotech Co., Ltd. for Human NRF1 (Nuclear respiratory factor 1) ELISA Kit