Fig 1: Effect of treatments with TBX3, SM, and the combination of both on the protein levels of Cx30 and TBX3 using ELISA analysis. Data showed overexpression of Cx30 and TBX3 protein in groups of treatments with TBX3, SM and TBX3 + SM as compared to cardiomyocytes group (p < 0.001). (TBX3: group that transfected with pcDNA TBX3, SM: group that added with small molecule SB431542, TBX3 + SM: Group that transfected with pcDNA TBX3 and added with with small molecule SB431542)
Fig 2: Result of immunofluorescence. It showed the green glow for the appearance of the TBX3 protein, and red glow for the appearance of Cx30 protein. Expression of TBX3 and Cx30 proteins showed in TBX3, SM, and TBX3 + SM group. The AD-MSCs and cardiomyocyte group did not show a positive glow. (TBX3: group that transfected with pcDNA TBX3, SM: group that added with small molecule SB431542, TBX3 + SM: Group that transfected with pcDNA TBX3 and added with with small molecule SB431542.)
Supplier Page from Fine Biotech Co., Ltd. for Human GJB6 (Gap junction beta-6 protein) ELISA Kit