RNAqueous-4PCR Total RNA Isolation Kit AM1914 from Thermo Fisher Scientific

Product Specs
ItemRNAqueous-4PCR Total RNA Isolation Kit
CompanyThermo Fisher Scientific
Price Inquire
Catalog NumberAM1914
Quantity30 Preps
ApplicationsRT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Sample TypeCells and tissues
Assay Time10 min. (After sample homogenization)
Yield≤10 µg per 1 mg tissue
≤1 µg per 1 x 10⁵ cells

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Cranial neural crest shortage leads to extensive craniofacial anomalies in mice mutant for the NR2F1/2 nuclear receptorsDevelopmental BiologyFebruary 1, 2026David Paulding, Simon J. Y. Han, Jonathan Timmons, Michelle Caye, Alexa Riedel, Samantha A. Brugmann, Lindsey Barskesequences were PCR-amplified from E8.5–10.5 mouse cDNA produced using the RNAqueous™-4PCR (Invitrogen AM1914) and High-Capacity cDNA Reverse Transcription (Applied Biosystems 4374966) kits. The products were cloned

(2) Short-Chain Fatty Acids Elicit Differential Expression of Growth Factors and Pro-Inflammatory Cytokines in Immortalized Rat Enteric Glial CellsNutrientsJanuary 29, 2026Michelle M. Beltran, Danielle M. Defriesextraction replicate per sample) using the RNAqueousTM-4PCR Kit (Invitrogen, Waltham, MA, USA; catalog #AM1914) according to the manufacturer’s instructions. RNA was eluted with two centrifugation steps, each with

(3) Glycolysis is intrinsically required in the follicle stem cell lineage but not in female germline stem cells or their early progeny in Drosophila melanogasterGENETICSJanuary 20, 2026Emily M. Wessel, Daniela Drummond-Barbosaremoval, 250 μl lysis buffer from the RNAqueous-4PCR Total RNA Isolation Kit (Thermo Fisher Scientific; #AM1914) were added, samples were homogenized using a motorized pestle, and RNA extraction proceeded according

(4) CXCR3 Deficiency Alleviates Retinal Ganglion Cell Loss by Regulating Neuron–Astrocyte Communication in a Mouse Model of GlaucomaInvestigative Ophthalmology & Visual ScienceNovember 17, 2025Fan Xia, Shuizhen Shi, Erick Palacios, Won-Kyu Ju, Hua Liu, Wenbo ZhangTRIzol reagent (15596018; Thermo Fisher Scientific), or from retinas using the RNAqueous-4PCR kit (AM1914; Thermo Fisher Scientific). RNA was then reverse transcribed into cDNA with the High-Capacity cDNA Reverse

(5) Maternal Exposure to Carbamazepine at Environmentally Relevant Concentrations Causes Growth Delay in Mouse EmbryosACS OmegaAugust 14, 2025Eliane Veretnik, Orit Douek-Maba, Rotem Kalev-Altman, Aluma Haiman, Maxim Quint, Vered Mordehay, Neta Shlezinger, Yuval Cinnamon, Benny Chefetz, Dalit Sela-Donenfelddifferent litters were used for this assay. mRNA was collected using an mRNA extraction kit (Ambion, no. AM1914, USA) according to the manufacturer’s protocol, along with the RNase-Free DNase Kit (Norgen Biotek,

(6) Stimulating Soluble Guanylyl Cyclase with the Clinical Agonist Riociguat Restrains the Development and Progression of Castration-Resistant Prostate CancerCancer ResearchJanuary 2, 2025Ling Zhang, Clara I. Troccoli, Beatriz Mateo-Victoriano, Laura Misiara Misiara Lincheta, Erin Jackson, Ping Shu, Trisha Plastini, Wensi Tao, Deukwoo Kwon, Xi Steven Chen, et al.cultured cells was extracted using the RNeasy (Qiagen, 74136) or RNAqueous-4PCR kit (Life Technologies, AM1914) as previously described (7). The following gene-specific TaqMan primer/probe sets were used: GUCY1A1

(7) Estrogen-dependent gene regulation: Molecular basis of TIMP-1 as a sex-specific biomarker for acute lung injuryPhysiological reportsSeptember 1, 2024Almuntashiri S, Dutta S, Zhu Y, Gamare S, Ramírez G, Irineo-Moreno V, Camarena A, Regino N, Campero P, Hernández-Cardenas CM, et al.real‐time quantitative PCR (qPCR) Total RNAs were isolated from cells using the RNAqueous Total RNA Isolation Kit (AM1914, Thermo Fisher Scientific). cDNA was generated by reverse transcription using the high‐capacity cDNA

(8) Sirt6 protects retinal ganglion cells and optic nerve from degeneration during aging and glaucomaMolecular TherapyJune 5, 2024Fan Xia, Shuizhen Shi, Erick Palacios, Wei Liu, Seth E. Buscho, Joseph Li, Shixia Huang, Gianmarco Vizzeri, Xiaocheng Charlie Dong, Massoud Motamedi, et al.in TriZol reagent (15596018; ThermoFisher Scientific) and RNA was isolated with RNAqueous-4PCR kit (AM1914; ThermoFisher Scientific). RNA concentration was measured using a NanoDrop (ThermoFisher Scientific)

(9) Taste receptor type 1 member 3 is required for the fertility of male miceHeliyonJanuary 30, 2024Woo-Jeong Shon, Hobin Seong, Jae Won Song, Dong-Mi Shinextraction, and the extracted RNA was purified using RNAqueous 4 PCR Kits (Ambion, Austin, TX, USA; AM1914) following the manufacturer's instructions. Prior to RNA sequencing, the quality and concentration of

(10) Auxin exposure disrupts feeding behavior and fatty acid metabolism in adult DrosophilaeLifeJanuary 19, 2024Sophie A Fleck, Puja Biswas, Emily D DeWitt, Rebecca L Knuteson, Robert C Eisman, Travis Nemkov, Angelo D'Alessandro, Jason M Tennessen, Elizabeth Rideout, Lesley N Weaveror kit,Invitrogen RNAqueous-4PCR DNA-free RNA isolation for RT-PCR kit,Thermo Fisher Scientific,Cat#AM1914, Commercial assay or kit,NEBNext Ultra II RNA Library Prep Kit for Illumina,New England Biolabs,Cat#

(11) A Mouse Model of MHV‐1 Virus Infection for Study of Acute and Long COVID InfectionCurrent ProtocolsOctober 23, 2023Andrew D. Masciarella, Dibe M. Di Gregorio, Rajalakshmi Ramamoorthy, Hussain Hussain, Arumugam R. Jayakumar, Michael J. Paidasfactors within bodily fluids and tissues. MATERIALS: Frozen samples RNAqueous®-4 PCR kit (cat# AM1914, Ambion, Austin, TX) High-Capacity cDNA reverse transcription kit (cat# 4368814, Applied Biosystems,

(12) GV1001 Inhibits the Severity of the Ligature-Induced Periodontitis and the Vascular Lipid Deposition Associated with the Periodontitis in MiceInternational journal of molecular sciencesAugust 8, 2023Kim SY, Kim YJ, Kim S, Momeni M, Lee A, Treanor A, Kim S, Kim RH, Park NH.Quantitative Real-Time Polymerase Chain Reaction Total RNA from mouse tissues was extracted using RNAqueous total RNA isolation kit (Invitrogen, AM1914, Carlsbad, CA, USA) and reverse-transcribed using iScript™ Reverse Transcription Supermix (Applied Biosystems,

(13) Gut taste receptor type 1 member 3 is an intrinsic regulator of Western diet-induced intestinal inflammationBMC medicineApril 28, 2023Shon WJ, Song JW, Oh SH, Lee KH, Seong H, You HJ, Seong JK, Shin DM.liquid nitrogen and stored at –80 °C until RNA extraction. Total RNA was isolated using RNAqueous (Cat# AM1914; Ambion, Austin, TX, USA), according to the manufacturer’s instructions. RNA extraction involved a DNase

(14) Silencing of long noncoding RNA MIAT inhibits the viability and proliferation of breast cancer cells by promoting miR-378a-5p expressionOpen Med (Wars)April 3, 2023Chao Yan, Yue Jincollected tissues were homogenized. Later, the total RNA was extracted using the Total RNA Isolation Kit (AM1914; Thermo Fisher) and miRNAs were extracted using the miRNA Isolation Kit (K157001; Thermo Fisher). After

(15) α-Mangostin Promotes In Vitro and In Vivo Degradation of Androgen Receptor and AR-V7 Splice Variant in Prostate Cancer CellsCancersApril 1, 2023Nauman MC, Won JH, Petiwala SM, Vemu B, Lee H, Sverdlov M, Johnson JJ.for 24 h. RNA was then extracted from the cells using an RNAqueous™-4PCR Total RNA Isolation Kit (#AM1914, Invitrogen, Waltham, MA, USA) by following the recommended protocol. RNA was resuspended in nuclease-free

(16) Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cellsnpj Regenerative MedicineMarch 25, 2023Alejandro M. Chibly, Vaishali N. Patel, Marit H. Aure, Mary C. Pasquale, Robert J. Morell, Daniel Martin Izquierdo, Erich Boger, Gemma E. Martin, Mousa Ghannam, Julianne Andrade, et al.extraction using the RNAqueous Micro Kit (invitrogen, Thermo Fisher Scientific, Vilnius, Lithuania #AM1914) following the manufacturer’s instructions. For staining, MECs were fixed with 4% PFA for 20 min at

(17) Mechanism of action deconvolution of the small-molecule pathological tau aggregation inhibitor Anle138bAlzheimer's Research & TherapyMarch 14, 2023Layla Hosseini-Gerami, Elena Ficulle, Neil Humphryes-Kirilov, David C. Airey, James Scherschel, Sarubini Kananathan, Brian J. Eastwood, Suchira Bose, David A. Collier, Emma Laing, et al.DIV3 plates were washed twice with DPBS and RNA extracted using Ambion RNAqueoskit (ThermoFisher, #AM1914). At DIV 7 and 14, the appropriate plates were extracted as described above. At DIV 14, a half media

(18) A human tau seeded neuronal cell model recapitulates molecular responses associated with Alzheimer’s diseaseScientific ReportsDecember 1, 2022Elena Ficulle et al.saline (DPBS) and RNA was extracted using the Ambion RNAqueous Total RNA isolation kit (Thermofisher, #AM1914). RNA concentration was determined with a NanoDrop spectrophotometer (Thermo Fisher) and quality was

(19) Long-Term Sequelae of COVID-19 in Experimental MiceMolecular neurobiologyOctober 1, 2022Paidas MJ, Cosio DS, Ali S, Kenyon NS, Jayakumar AR.RT-qPCR Isolation of mRNA was performed as previously described [46, 47]. In brief, the RNAqueous®-4PCR kit (#AM1914; Ambion, Austin, TX, USA) was used for the RNA isolation. High-Capacity cDNA Reverse Transcription Kit

(20) Pro-inflammatory cytokines mediate the epithelial-to-mesenchymal-like transition of pediatric posterior fossa ependymomaNature communicationsJuly 8, 2022Aubin RG, Troisi EC, Montelongo J, Alghalith AN, Nasrallah MP, Santi M, Camara PG.were imaged and RNA extraction was carried out using the RNAqueous 4PCR kit (Life Technologies, cat. #AM1914). RNA was immediately stored at −80 °C until cDNA synthesis. cDNA was synthesized using Maxima H Minus

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RNAqueous-4PCR Total RNA Isolation Kit from Thermo Fisher Scientific

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RNAqueous-4PCR Total RNA Isolation Kit from Thermo Fisher Scientific

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