Fig 2: Relationship network for the selected circadian lock differentiation proteins generated in the STRING database. (CLOCK, clock circadian regulator; PRKAA1, protein kinase AMP-activated catalytic subunit alpha 1; PRKAA2, protein kinase AMP-activated catalytic subunit alpha 2; PRKAB1, protein kinase AMP-activated non-catalytic subunit beta 1; PRKAB2, protein kinase AMP-activated non-catalytic subunit beta 2; PER1, period circadian regulator 1; PER2, period circadian regulator 2; PER3, period circadian regulator 3. The green line for interaction indicates “textmining”, the black line “co-expression”, the turquoise line “interactions from curated databases”, the pink line “interactions experimentally determined”, the blue line “gene co-occurrence”, and the violet line “protein homology”).

Fig 3: The degree of methylation of selected genes in the G2, G3, and G4 astrocytic tumor samples. (CLOCK, clock circadian regulator; PRKAA1, protein kinase AMP-activated catalytic subunit alpha 1; PRKAA2, protein kinase AMP-activated catalytic subunit alpha 2; PRKAB1, protein kinase AMP-activated non-catalytic subunit beta 1; PRKAB2, protein kinase AMP-activated non-catalytic subunit beta 2; PER1, period circadian regulator 1; PER2, period circadian regulator 2; PER3, period circadian regulator 3; G2—II grade; G3—III grade; G4—IV grade).

Fig 4: Changes in the expression profile of genes differentiating tumor samples compared to control tissues obtained by the microarray analysis (−2.0 < FC > 2.0; p < 0.05). (CLOCK, clock circadian regulator; PRKAA1, protein kinase AMP-activated catalytic subunit alpha 1; PRKAA2, protein kinase AMP-activated catalytic subunit alpha 2; PRKAB1, protein kinase AMP-activated non-catalytic subunit beta 1; PRKAB2, protein kinase AMP-activated non-catalytic subunit beta 2; PER1, period circadian regulator 1; PER2, period circadian regulator 2; PER3, period circadian regulator 3; FC, fold change; G2—II grade; G3—III grade; G4—IV grade. Data are presented as the mean ± standard deviation).