Description
This Human Pregnancy-Specific Beta 1 Glycoprotein 1 (CD66f) ELISA Kit is intended for quantitative detection of human PSG1 in cell culture supernates, serum, plasma (heparin, EDTA) and urine. Strip well format. Reagents for up to 96 tests.
This human PSG1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PSG1 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: Q35-P419) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PSG1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: The PSG1 is a semi-automatic sniper rifle, Pregnancy-specific beta-1 glycoprotein 1 (PSG-1) is also known as SP1, PSbG1, or B1G1, and is designated CD66f. It is mapped to 19q13.2. The human placenta is a multihormonal endocrine organ that produces hormones, enzymes, and other molecules that support fetal survival and development. Pregnancy-specific beta-1-glycoprotein (PSBG, PSG) is a major product of the syncytiotrophoblast, reaching concentrations of 100 to 290 mg/l at term in the serum of pregnant women. PSG is a member of the immunoglobulin (Ig) superfamily