Description
This Rat Angiopoietin-Related Protein 7 ELISA Kit is intended for quantitative detection of rat ANGPTL7 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This rat ANGPTL7 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL7 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: Q22-P337) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat ANGPTL7 amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to chromosome: 5. Angiopoietin-like Protein 7 (ANGPTL7), also known as cornea-derived transcript 6 (CDT6), is a secreted 45 kDa protein that contains a coiled-coil domain (aa 30-110) and a fibrinogen-like domain (aa 113-334). ANGPTL7 is expressed in the stromal layer of the cornea, and it forms disulfide-linked homotetramers. It contributes to corneal morphogenesis by suppressing angiogenesis and promoting the deposition of extracellular matrix components. ANGPTL7 is up-regulated in glaucoma and accumulates in the aqueous humor. Mature mouse ANGPTL7 shares 88% and 98% sequence identity with human and rat ANGPTL7