Rat TNF-alpha Quantikine ELISA Kit RTA00-1 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRat TNF-alpha Quantikine ELISA Kit
CompanyR&D Systems, a Bio-Techne Brand
Price $680.00Supplier Page View Company Product Page
Catalog NumberRTA00-1
Quantity1 Kit (1 Plate) (also 1 Pack (50 Plates), 1 Pack (6 Plates))
ApplicationsELISA
Detection Range12.50 - 800 pg/mL
ReactivityRat
Sample TypeCell Culture Supernates (17 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity5 pg/mL
Detection TargetTNF-alpha
Assay Time4.5 hours
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesRATTNF, TNF-alpha, Tnfa

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Melittin Nanoparticles Mitigate Glyphosate-Induced Nephrotoxicity via Cytokine Modulation and Bax/Nrf2 PathwaysBiomedicinesOctober 24, 2025Amany M. Hamed, Zeyad Elsayed Eldeeb Mohana, Azza M. A. Abouelella, Wafaa A. Abdellah, Dalia A. Elbahy, Noha A. R. Fouda, Dina M. Monir, Safaa S. Soliman, Ahmed Mohamed Mahmoud Abdelfattah Elkassas, Elsayed Eldeeb Mehana Hamouda, et al.to the provided protocols. Pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-α; Cat. No. RTA00-1) and interleukin-6 (IL-6; Cat. No. R6000B), were analyzed using ELISA kits from R&D Systems (Shanghai,

(2) Ovary Metal Toxicity Remediation by Agro-Food Waste: Evidence for a Regulatory Mechanism of Oxidative Stress by Banana (Musa cavendish) Peel ExtractAntioxidants (Basel)September 18, 2025Boma F. Eddie-Amadi, Rubina Vangone, Valeria Guerretti, Harrison A. Ozoani, Kenneth O. Okolo, Dokubo Awolayeofori, Tamuno-Boma Odinga-Israel, Kpobari W. Nkpaa, Emidio M. Sivieri, Orish E. Orisakwe, et al.E-EL-R0015, Elabscience Biotechnology Company, Beijing, China), tumor necrosis factor alpha (TNF-α; Cat no.: RTA00-1, R&D Systems, Elabscience Biotechnology Company, Beijing, China), caspase-3 (Cas-3; Cat. no.: E-EL-R0160,

(3) Targeting PFKFB3 to restore glucose metabolism in acute pancreatitis via nanovesicle deliveryMol MedJuly 5, 2025Hai Jiang, Zhipeng Xu, Qi Song, Junjie Tao, Jia Liu, Qiang Wang, Huaisheng Zhang, Heng Zhu, Qiliang Li, Lei Lifor 10 min. The assays were conducted using commercial ELISA kits (IL-1β: RLB00-1, IL-6: R6000B, TNF: RTA00-1, R&D Systems, USA) following the manufacturer’s instructions. First, 200 µL of coating buffer containing

(4) Precision Nanotherapy for Spinal Cord Injury: Modulating SLC16A3 With Methylprednisolone-Loaded NanoparticlesNeurospineJune 30, 2025Jianwei Lv, Shibo Ma, Duo Shanrpm, 10 minutes) to separate the serum. A commercial ELISA kit (IL-1β: RLB00-1, IL-6: R6000B, TNF: RTA00-1, R&D Systems) was used following the manufacturer’s instructions. The capture antibody was coated on

(5) Irisin and liraglutide combination therapy mitigates myocardial reperfusion injury in obese rats: Role of endoplasmic reticulum stress and apoptosisArchives of biochemistry and biophysicsJune 1, 2025Ma T, Wang J, Sun G, Li K, Qu H, Wang Y, Li S, Wu B.

(6) Modulation of AMPK by esomeprazole and canagliflozin mitigates methotrexate-induced hepatotoxicity: involvement of MAPK/JNK/ERK, JAK1/STAT3, and PI3K/Akt signaling pathwaysNaunyn-Schmiedeberg's Archives of PharmacologyMarch 7, 2025Ahmed M. El-Dessouki, Mohamed E. Kaml, Mohammed F. EL-Yamanytissues were quantitatively assessed using ELISA kits. These kits, obtained from R&D Systems (Cat. No. RTA00-1 for TNF-α, Minneapolis, USA) and Elabscience (Cat. No. EL-R0015 for IL-6, Houston, USA), were employed

(7) L-arginine administration exacerbates myocardial injury in diabetics via prooxidant and proinflammatory mechanisms along with myocardial structural disruptionWorld Journal of DiabetesFebruary 15, 2025Rasha A Mansouri, Esam M Aboubakr, Huda F Alshaibi, Adel M Ahmedfollowing the manufacturer's instructions. Catalog numbers for the kits are DY401-05 for IL-1β and RTA00-1 for TNF-α. Determination of cardiac catalase activity The breakdown of H2O2 by the catalase (CAT)

(8) Tabernaemontana stapfiana Britten (Apocynaceae) Stem Bark Prevents Alcl(3)-Induced Cognitive Disability: Antioxidant and Anti-Inflammatory Activities in Wistar RatsOxidative medicine and cellular longevityJanuary 1, 2025Ngenteh ML, Sanda KA, Neng TP, Fubi BBL, Nchang NR, Nsah KB, Mahamat O.

(9) Deep brain stimulation mitigates memory deficits in a rodent model of traumatic brain injuryBrain stimulationNovember 1, 2024Rabelo TK, Campos ACP, Almeida Souza TH, Mahmud F, Popovic MR, Covolan L, Betta VHC, DaCosta L, Lipsman N, Diwan M, et al.

(10) Development of a proliposomal pretomanid dry powder inhaler as a novel alternative approach for combating pulmonary tuberculosisInternational journal of pharmaceuticsOctober 25, 2024Aekwattanaphol N, Das SC, Khadka P, Nakpheng T, Ali Khumaini Mudhar Bintang M, Srichana T.

(11) N-Pep-Zn Improves Cognitive Functions and Acute Stress Response Affected by Chronic Social Isolation in Aged Spontaneously Hypertensive Rats (SHRs)BiomedicinesOctober 4, 2024Stepanichev MY, Onufriev MV, Moiseeva YV, Nedogreeva OA, Novikova MR, Kostryukov PA, Lazareva NA, Manolova AO, Mamedova DI, Ovchinnikova VO, et al.

(12) Efficacy of Thymoquinone and Hesperidin in Attenuating Cardiotoxicity from 5-Fluorouracil: Insights from In Vivo and In Silico StudiesToxicsSeptember 23, 2024Farooq J, Sultana R, James JP, Fathima C Z, Almutairy AF, Hussain ASM.

(13) Royal Jelly Exerts a Potent Anti-Obesity Effect in Rats by Activating Lipolysis and Suppressing AdipogenesisNutrientsSeptember 19, 2024Alaa Hasanain Felemban, Ghedeir M. Alshammari, Abu ElGasim Ahmed Yagoub, Ali Saleh, Mohammed Abdo YahyaAfter 24 h, the medium was analyzed for TNF-α and IL-6 levels using ELISA kits (Cat. #R6000B and Cat. #RTA00-1, R&D System, MN, USA). Analysis was conducted in triplicate for n = 6 samples/group. 2.10. Protein Extraction

(14) Role of Anonychium africanum (Plantae, Fabaceae) in Metal Oxido-Inflammatory Response: Protection Evidence in Gonad of Male Albino RatAntioxidants (Basel)August 24, 2024Harrison A. Ozoani, Orish Ebere Orisakwe, Costantino Parisi, Loredana Assisi, Anthonet N. Ezejiofor, Kenneth O. Okolo, Chinna N. Orish, Rubina Vangone, Emidio M. Sivieri, Giulia GuerrieroElabscience Biotechnology Company, Beijing, China), and tumor necrosis factor alpha (TNF-α; Cat no.: RTA00-1, R&D Systems, Elabscience Biotechnology Company, Beijing, China) were detected in the testis of rats

(15) A Novel Model of Venovenous Extracorporeal Membrane Oxygenation in Rats with Femoral Cannulation and Insights into Hemodynamic ChangesBiomedicinesAugust 10, 2024Edinger F, Zajonz T, Mayer N, Schmidt G, Schneck E, Sander M, Koch C.

(16) Geranylgeranylacetone mitigates sepsis-associated intestinal injury through CHIP-dependent anti-inflammation and anti-oxidative effectInternational immunopharmacologyJune 30, 2024Liu X, Liu Y, Su X, Jiang L, Tang G, Wang Y.

(17) New Insights into Hepatic and Intestinal Microcirculation and Pulmonary Inflammation in a Model of Septic Shock and Venovenous Extracorporeal Membrane Oxygenation in the RatInternational journal of molecular sciencesJune 16, 2024Edinger F, Zajonz T, Holtz L, Schmidt G, Schneck E, Sander M, Koch C.

(18) Asiatic acid rescues intestinal tissue by suppressing molecular, biochemical, and histopathological changes associated with the development of ulcerative colitisBioscience reportsMay 29, 2024Lokman MS, Kassab RB, Salem FAM, Elshopakey GE, Hussein A, Aldarmahi AA, Theyab A, Alzahrani KJ, Hassan KE, Alsharif KF, et al.

(19) Pioglitazone treatment mitigates cardiovascular bioprosthetic degeneration in a chronic kidney disease modelFrontiers in pharmacologyJanuary 1, 2024Katahira S, Barth M, Döpp R, Sugimura Y, Schmidt V, Selig JI, Saiki Y, Jankowski J, Marx N, Jahnen-Dechent W, et al.

(20) Neuroprotective effects of a novel peptide through the Rho-integrin-Tie2 and PI3K/Akt pathways in experimental autoimmune encephalomyelitis modelFrontiers in pharmacologyJanuary 1, 2024Zhou W, Qu H, Fu XX, Xu MM, Li Q, Jiang Y, Han S.

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Rat TNF-alpha Quantikine ELISA Kit

TNF-alpha ELISA

Rat TNF-alpha Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Rat TNF-alpha Quantikine ELISA Kit from R&D Systems, a Bio-Techne Brand

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