Description
Inclusion Body Protein Isolation Micro Kit?is designed for the purification of recombinant proteins expressed in bacteria as inclusion bodies. Bacteria are widely used for the expression of differentrecombinant?protein. However, 70-80% of the proteins expressed in?E. coli?by recombinant techniques are contained in inclusion bodies (i.e., protein aggregates). The kit includes reagents specially formulated to achieve rapid and high-quality purification of inclusion body proteins using three processes: 1. Lysis of bacterial cells to release inclusion bodies in solid form2. Solubilization of purified inclusion bodies3. Purification of the recombinant protein using spin column chromatography