Description
Principle of the Assay: Glutathione is a Tripeptide of Glycine, Glutamic acid and Cysteine. In the red blood cell, reduced Glutathione (GSH) is the key to maintain hemoglobin in reduced state and protect cells from Oxidative damage. GSH is the most important antioxidant sulfhydryl compound in cells, which plays an important role in oxidation resistance, protein sulfhydryl protection and amino acid transport across membrane. The ratio between reduced and oxidized Glutathione (GSH/GSSG) is the main indicator of the cell's redox state. Therefore, measuring the content of GSH and GSSG and the ratio of GSH/GSSG in cells can reflect the redox state of cells. CheKine™ Total Glutathione (T-GSH) Colorimetric Assay Kit can detect samples of Animal and Plant Tissues, Bacteria, Cells, Serum, etc. Glutathione reductase (glutathione reductase, GR) is used to reduce GSSG to GSH. GSH can react with 5,5'-Dithiobis-(2-Nitrobenzoic Acid) (5,5'-Dithiobis-2-Nitrobenzoic Acid, DTNB) to form 2-Nitro-5-Diol Benzoic Acid. This substance is yellow and has a maximum light absorption at a wavelength of 412 nm. In this way, the total glutathione content in the sample can be determined