Description
Principle of the Assay: Triglycerides (TG) are fat molecules formed by long-chain Fatty acids and Glycerol. They are not only the main components of cell membranes, but also important respiratory substrates. Serum triglycerides (TG) is an important index for clinical blood lipid measurement. CheKineTM Triglyceride (TG) Colorimetric Assay Kit provides a simple method for detecting TG concentration in a variety of biological samples such as Serum, Plasma, Animal and Plant Tissues, Cells, Cell Supernatant, Bacteria. In the assay, TG can be extracted by Isopropanol and then saponified by KOH to produce Glycerol and Fatty Acid. Further, periodic Acid Oxidizes Glycerin to form Formaldehyde. In the presence of Chloride Ions, Formaldehyde can react with Acetylacetone to form a yellow substance which has a characteristic absorption peak at 420 nm. The Triglyceride (TG) present in the sample is proportional to the signal obtained