Description
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for NSE has been precoated onto a microplate. Standards and samples are pipetted into the wells and any NSE present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for NSE is added to the wells and binds to the combination of capture antibody-NSE in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of NSE present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven NSE standard dilutions and NSE sample concentration determined