Human tonsil stained with with OriGene Mouse anti MS4A1 (UM800009 diluted 1:600) seen as the blue membrane stain HIER buffer TEE (B21C-100) was used 10 minutes pressure cooker. Secondary Polink1 anti-Ms D18-100, and AP Blue chromogens was mix prior to placing on tissue for 10 minutes.
Supplier Page from OriGene Technologies for AP-Blue Chromogen Kit