Fig 1: Overexpression of IGFBP-rP1 affects the polarization of EC-related macrophages by regulating the PI3K/AKT pathway. Human THP-1 monocytes were treated with PMA for 24 h to induce M0 macrophages. Then, M0 cells were co-cultured with EC cells overexpressing IGFBP-rP1 and/or overexpressing AKT for 48 h to produce TAMs. The level of M1 markers, (A) iNOS and (B) TNF-α and M2 markers, (C) CD163, (D) Arg-1 and (E) MR were analyzed using ELISA. *P<0.05. IGFBP-rP1, insulin-like growth factor binding protein-related protein 1; EC, endometrial carcinoma; TAMs, tumor-associated macrophages; iNOS, inducible nitric oxide synthase; NC, negative control; Arg-1, arginase-1; MR, mannose receptor.
Fig 2: Correlations between IL-10, RANTES, CD206 CD163, FAP, Arginase 1, and TGF-β1 with the soluble immune checkpoint molecules.
Supplier Page from Abcam for Human CD163 ELISA Kit