Fig 1: BTG3 siRNA transfection abolishes the miR-18a-5p inhibitor influences on inflammatory cytokine release in HFLSs. After transfection, the secretion of inflammatory cytokines, such as (a) TNF-a, (b) IL-1β, and (c) IL-6, and MMPs including MMP1 (d) and MMP3 (e) were determined via ELISAs in different groups. **P < 0.01 vs. inhibitor control; ##P < 0.01 vs. miR-18a-5p inhibitor + control siRNA. miR, microRNA; BTG3, B-cell translocation gene 3. Experiments were repeated for three times.
Fig 2: miR-18a-5p mimic abrogates lncRNA CASC2 effects on inflammatory factor release in HFLSs. After transfection, the excretion of inflammatory cytokines, including (a) TNF-α, (b) IL-1β, and (c) IL-6, and MMPs including MMP1 (d) and MMP3 (e) were analyzed via ELISAs in different groups. **P < 0.01 vs. control plasmid; ##P < 0.01 vs. lncRNA CASC2 plasmid + mimic control. miR, microRNA; lncRNA CASC2, long non-coding RNA cancer susceptibility candidate 2. Experiments were repeated for three times.
Fig 3: The flow of the assay. KOA: knee osteoarthritis. ESWT: extracorporeal shock wave therapy. EESWT: electroacupuncture combined with extracorporeal shock wave therapy. LKSS: lysholm kness. VAS: visual analogue scale. WOMAC: Western Ontario and McMaster Universities Arthritis Index. ROM: range of motion. NO: nitric oxide. SOD: superoxide dismutase. IL-1β: Interleukin-1β. TNF-α: tumor necrosis factor α. MMP-3: matrix metalloproteinase-3. TGF-β1: transforming growth factor-β1.
Fig 4: Changes in the expression levels of NO, SOD, IL-1β, TNF-α, MMP-3 and TGF-β1 in synovial fluid before and after treatment. a–f: NO, SOD, IL-1β, TNF-α, MMP-3 and TGF-β1, respectively. **p < 0.01, compared with before treatment. ##p < 0.01, compared with Control group. EESWT, electroacupuncture combined with extracorporeal shock wave therapy. ESWT, extracorporeal shock wave therapy. NO: nitric oxide. SOD: superoxide dismutase. IL-1β: Interleukin-1β. TNF-α: tumor necrosis factor α. MMP-3: matrix metalloproteinase-3. TGF-β1: transforming growth factor-β1.
Fig 5: The flow of the assay. KOA: knee osteoarthritis. R-ESWT: radial extracorporeal shock wave therapy. LESWT: Low-intensity pulsed ultrasound combined with radial extracorporeal shock wave therapy. LKSS: lysholm kness. VAS: visual analogue scale. WOMAC: Western Ontario and McMaster Universities Arthritis Index. ROM: range of motion. NO: nitric oxide. SOD: superoxide dismutase. IL-1β: Interleukin-1β. TNF-α: tumor necrosis factor α. MMP-3: matrixmetalloproteinase-3. TGF-β1: transforming growth factor-β1
Supplier Page from Abcam for Human MMP3 ELISA Kit