Fig 1: CD200R functions as an inhibitory receptor on T cells after ligation by CD200-Fc, and its function is not influenced by soluble CD200his. A, B: Cytokine production in CD4+ T cells (A) and CD8+ T (B) cells after stimulation with anti-CD3 (or isotype), and CD200R ligation by CD200-Fc (or control), measured with flowcytometry. Statistical significance was determined by Wilcoxon matched-pairs signed rank test, n = 8 C: Cytokine production in supernatant from CD3+ T cells after stimulation with anti-CD3 (or isotype), and CD200R ligation by CD200Fc (or control), measured with ELISA, n = 6. D, E: Cytokine production in CD4+ T cells (D) and CD8+ T cells (E) after stimulation with anti-CD3/CD28 (or isotype), and CD200R ligation by CD200Fc (or control), measured with flowcytometry. CD200Fc and CD200his were added soluble. Statistical significance was determined with a Wilcoxon matched-pairs singed rank test, n = 13. F, G: Expression of IL-2 and IFN-γ in CD4 + T cells (F, n = 4) or CD8 + T cells (G, n = 4) activated with anti-CD3/anti-CD28 T cell activation beads (activation beads) in presence of CHO-CD200 or parental CHO cells. Statistical significance was determined with a Wilcoxon matched-pairs singed rank test
Fig 2: CD200R functions as an inhibitory receptor on T cells in patients with systemic autoimmune diseases and is not affected by type I IFN. A: cytokine production in CD4+ and CD8+ T cells after stimulation with anti-CD3/CD28 beads, and CD200R ligation with Ox108. Statistical significance was determined with a mixed-effects analysis and Šídák’s post-hoc, or unpaired-T test. Contols: n = 3, SLE: n = 5. B: The effect of 100 units of IFNα on cytokine production in CD4+ and CD8+ control T cells after stimulation with anti-CD3/CD28 activating beads, and CD200R ligation with a CD200 expressing CHO cell line. Statistical significance was determined with a mixed-effects analysis and Šídák’s post-hoc, or unpaired-T test, n = 5. SLE: systemic lupus erythematosus; CHO: Chinese hamster ovary cell line
Fig 3: CD200R membrane expression on T cells is increased in SLE patients. A: Flowcytometry gating strategy for CD200R and CD200 in CD3+ T cells in representative patient with SLE (solid line) and control (dotted line). The number in the histograms depict the % positive cells for the SLE example (purple, upper) and the Control example (gray, lower). B: % of CD200 and CD200R expressing CD3+ T cells in controls and patients with systemic autoimmune disease. Differences were calculated with a Kruskal Wallis test. The squares in Control and SLE indicate which donors are plotted in the example histograms in A. SLE: systemic lupus erythematosus; pAPS: primary antiphospholipid syndrome; pSS: primary Sjögren's syndrome; SSc: systemic sclerosis
Supplier Page from Abcam for Human CD200R ELISA Kit (CD200R1)