Fig 1: Effect of ADAMTS1 knock down on known ADAMTS1 regulated genes. A The mRNA expression of genes associated with ADAMTS1 regulation (L1CAM, FBLN1 and SDC4) was deduced by qRT-PCR after 48 h post transfection of ADAMTS1 siRNA in OVCAR4, OVCAR5 and ES2 cell lines as described in Methods. Graphs represent amount of mRNA relative to 18S ± SEM derived from three experiments done in triplicate. P indicates parental cell line treated with transfection reagent, C are cells transfected with scrambled siRNA and A-KD is a representative of a pool of all three ADAMTS1 siRNAs knock down cells. Significance is indicated by p < 0.05 and **p < 0.01, one-way ANOVA using Tukey’s multiple comparison test. B Secreted Syndecan-4 (SCD4) conditioned media levels were deduced by commercially available ELISA, as described in Methods. Graphs represent mean of total ± SEM, (n = 3). Significance is indicated by * p < 0.05 and ** p < 0.01, one-way ANOVA using Tukey’s multiple comparison test