Fig 1: Cytokines + bFGF.Growth factor analysis of spent media from cultures of three cultivated meat-relevant cell types. A preliminary screening of 30 bovine cytokines was performed using a multiplex enzyme-linked immunosorbent assay (ELISA) to compare the concentrations in the spent media samples at three time points over 3 weeks. The five cytokines with the most significant changes over time were interferon gamma-induced protein 10 (IP-10), basic fibroblast growth factor (bFGF), insulin-like growth factor 1 (IGF-10), neural cell adhesion molecule 1 (NCAM-1), and decorin. The data for these proteins are plotted for the samples from (a) primary embryonic chicken muscle fibroblasts (cMFB), b primary embryonic chicken muscle precursor cells (cMPC), and c the C2C12 murine myoblast-like cell line. Data points represent the mean of two biological replicates ± standard deviation. Statistical analysis for this preliminary screening was performed using two-way analysis of variance (ANOVA) followed by the Holm-Šídák post-test for multiple comparisons with a = 0.05 (ns, not significant. *p < 0.05. **p < 0.01. ****p < 0.0001). It should be noted that statistical power was limited by having up to only two biological replicates per time point, but overall trends can be discerned in many cases despite lack of statistical significance. d Further analysis of basic fibroblast growth factor (bFGF) was performed using a standard ELISA and concentrations are shown with each cell type overlaid. Dotted lines represent the measured concentrations of bFGF in fresh medium that was prepared immediately before analysis as well as in Matrigel stock solution.