Fig 1: Oxidative Stress Markers and Inflammatory Factors in Rat Serum Following Different Vasectomy Surgeries. Note: (A) Serum serotonin (5-HT) levels: The 5-HT levels in the MV group were lower than those in the traditional vasectomy group. B Serum malondialdehyde (MDA) levels: The MDA levels in the testicular and epididymal tissues of the modified vasectomy group were lower than those in the traditional vasectomy group. C Serum superoxide dismutase (SOD) activity: The SOD activity in the testicular and epididymal tissues of the modified vasectomy group was higher than that in the traditional vasectomy group. (D-F) Serum interleukin-1 (IL-1), interleukin-2 (IL-2), and interleukin-6 (IL-6) levels: The serum levels of IL-1, IL-2, and IL-6 were lower in the modified vasectomy group compared to the traditional vasectomy group, reflecting more severe tissue damage and inflammatory responses in the traditional vasectomy group. (*P < 0.05 vs. sham operation; #P < 0.05 vs. traditional vasectomy). Statistical analyses were performed using SPSS 20.0 software. Each group included six rats, and quantitative results are presented as X ± S. Independent sample t-tests were used for comparisons between groups. GraphPad Prism 6 was used to create statistical graphs. The significance level was set at 0.05. Group definitions: SO, sham operation group; NC, negative control group; TV, traditional vasectomy group; MV, modified vasectomy group
Fig 2: Baicalein reduces IL-1β and IL-18 secretion and limits NF-κB activation in H9c2 cells A and B, ELISA quantification of IL-1β and IL-18 secretion. Supernatants collected after 24 h of lipopolysaccharide (LPS) stimulation were analyzed using rat IL-1β (ab255730) and IL-18 (ab213909) ELISA kits (Abcam). Plates were read at 450 nm. C, NF-κB pathway Western blotting. Total and phosphorylated p65 (Ser536) and IκBα were evaluated by Western blot using primary antibodies (CST) and quantified by densitometry. Band intensities were normalized to total p65 for phosphorylated p65 and GAPDH for IκBα.
Supplier Page from Abcam for Rat IL-1 beta ELISA Kit