Fig 1: (A-B) Changes in bone metabolism biomarkers, bone microstructure and related protein expression in three groups of mice after CBT intervention. (C-D) Representative images of ABH staining (40× scale bar: 1000 μm, 100× scale bar: 400 μm) (E) Analysis of the distal femoral epiphyseal region of mice using micro-computed tomography (CT). (F-G) Representative images of Immunohistochemistry of RANKL and OPG. (H-K) Comparison of BV/TV, Tb.Th, Tb.N, Tb.Sp. (L) Protein blot analysis of bone tissue targeting proteins associated with the RANKL/OPGpathway and inflammatory factors. (M-P) Expression of RANKL, TNF-α, OPG, and TGF-β1 proteins in bone tissues. n = 12, *P < 0.05, **P < 0.01, ***P < 0.001.