Description
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Glycated Hemoglobin A1c (HbA1c). A competitive inhibition reaction is launched between biotin labeled Glycated Hemoglobin A1c (HbA1c) and unlabeled Glycated Hemoglobin A1c (HbA1c) (Standards or samples) with the pre-coated antibody specific to Glycated Hemoglobin A1c (HbA1c). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glycated Hemoglobin A1c (HbA1c) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Glycated Hemoglobin A1c (HbA1c) level in the sample or standard