Description
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Nuclear was pre-coated onto 96-well plates. And the Biotin- labeled Nuclear was used as detection antigen. The standards, test samples and Biotin- labeled Nuclear were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the anti-nuclear antibody amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of anti-nuclear antibody can be calculated