Fig 1: Confirmation of DEGs at the protein level. (A) Collagen XXIII was upregulated in fibroids regardless of compression status. Representative images of collagen XXIII with DAPI nuclear stain in fibroid and myometrial cells are shown, Scale bar = 30 μm. (B) Image analysis of collagen XXIII. n=4 biological replicates with four technical replicates each. (C) EphB1 content per μg DNA. (D–G) DEGs that were also identified as DEPs. (D) 23 Fibroid DEGs upregulated with compression. Red indicates genes associated with IL-17 pathway enrichment (q = 5.95 × 10−5). Purple indicates genes associated with uterine disease enrichment (q = 0.0070). (E) 8 Fibroid DEGs downregulated with compression. Red indicates genes associated with collagen-containing ECM enrichment (q = 0.0083). (F) Compression DEGs upregulated in fibroids. (G) Compression DEGs were downregulated in fibroids. Red indicates genes associated with the regulation of smooth muscle cell-matrix adhesion enrichment (q = 0.0197). *Indicates P<.05. DAPI = 4’,6-diamidino-2-phenylindole; DEGs = differentially expressed genes; ECM = extracellular matrix.