Fig 1: Immunofluorescence localization and expression of G-CSF in control group and mouse model of periodontitis. Images of the gingival junctional epithelium were taken by hematoxylin–eosin staining (A, B, E, F) with 20 × and 40 × objectives. The membranes of positive cells exhibited green fluorescence (C, G), while the nuclei exhibited blue fluorescence (D, H). Control group (A to D), periodontitis group (E to H), merged images (D, H). C, D, G and H: Bars, 20um. D, dentin; GE, gingival epithelium; CT, connective tissue; AB, alveolar bone
Fig 2: ELISA data showing levels of G-CSF in serum and bone marrow in control and mouse model of periodontitis. A significantly greater level of G-CSF in serum from the periodontitis group, compared with the control group (A). The amount of G-CSF in bone marrow tended to be upregulated in the periodontitis group, but there was no difference between the two groups (B). Data are presented as mean ± SD. *P < 0.05 using two-tailed Students t-test. ns, no differences
Supplier Page from Wuhan Fine Biotech Co., Ltd. for Mouse G-CSF(Granulocyte Colony Stimulating Factor) ELISA Kit