LunaScriptRT SuperMix Kit E3010S from New England Biolabs

Product Specs
ItemLunaScriptRT SuperMix Kit
CompanyNew England Biolabs
Price $152.00 Inquire
Catalog NumberE3010S
Quantity25 reactions
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Transcriptional response of spermathecal secretory cells to mating and receipt of seminal fluid proteins in Aedes aegyptiiScienceJune 19, 2026Claudia A.S. Wyer, Mehrnaz Afkhami, David A. Ellis, Sylvie Pitcher, I. Alexandra Amaro, Patrick K. Perish, Jake W. Angelico, Emilie M. Gray, Yasir H. Ahmed-Braimah, Mariana F. Wolfner, et al.Nuclei Isolation Kit: Nuclei PURE Prep,Sigma-Aldrich,NUC201 TURBO DNA-free Kit,Life Technologies,AM1907 Lunascript RT SuperMix,NEB,E3010S CloneAmp HiFi PCR premix,Takara,639298 Monarch PCR cleanup kit,NEB,T1030 TranscriptAid T7 High Yield Transcription Kit

(2) Reverse genetics approach for arteriviruses using circular polymerase extension reactionAccess MicrobiolMay 21, 2026Rabab T. Hassanien, Wellesley Dittmar, Udeni B. R. Balasuriya, GuanQun Liu, Côme J. Thieulent, Samah Eid, Mariano Carossinoreverse transcribed to cDNA using the LunaScript® RT SuperMix Kit (NEB, Ipswich, MA, USA, Cat. No. E3010S), which contained both oligo(dT) and random hexamer primers. The reverse transcription reaction was

(3) Rapid and precise amplicon-based genome sequencing to preparedness and response chikungunya virus outbreaksEmerg Microbes InfectApril 15, 2026Kaiying Wang, Jinhui Li, Tianyuan Liu, Peihan Li, Ning Wang, Yuqi Liu, Yufeng Liang, Fenxiang Li, Chuhong Wei, Huacheng Yan, et al.for pool 1 and S2 for pool 2. Targeted amplification of CHIKV genome The LunaScript® SuperMix (NEB, E3010S) was used for cDNA synthesis. We mixed RNA Sample 16 µL and LunaScript RT SuperMix (5X) 4 µL together

(4) Rational engineering of the P5 TRS-mimic site and REP78/68 start codon yields promoter variants that improve rAAV purity while maintaining high titersMolecular Therapy AdvancesMarch 12, 2026Stephen M. Winston, Mark A. Brimble, Kristin B. Wiggins, Isaiah L. Reeves, Yunyu Spence, Baochang Fan, Shaoyuan Tan, Christopher L. Morton, Pei-Hsin Cheng, Mary Y. Spence, et al.transferred to a 200-μL PCR tube containing LunaScript RT SuperMix mastermix (New England Biolabs, cat. no. E3010S). The tube was placed in a thermocycler for 2 min at 25°C, 10 min at 55°C, and 1 min at 95°C. cDNA was

(5) RT-qPCR and split-luciferase assays enable batch standardization and analysis of engineered virus-like particle transductionMolecular Therapy AdvancesMarch 12, 2026Lucia Nicosia, Joss B. Murray, Emma Collins, Lisa Lonetti, Patrick T. Harrison, Martina F. ScallanBE-eVLP RNA were reverse transcribed with random hexamers using LunaScript RT SuperMix Kit (NEB, Cat#E3010S) for first-strand cDNA synthesis or added to no-RT controls, according to the manufacturer’s protocols. qPCR

(6) Camsap2a regulates actomyosin flow and Rab5ab-mediated macropinocytosis in the yolk cell during zebrafish epibolyDevelopmentFebruary 1, 2026Haoyu Wan, Sifa Quibria, Ernest Iu, Sirma Damla User, Sergey V. Plotnikov, Ashley E. E. Brucemanufacturer's instructions; 1 µg total RNA was used to make cDNA with the LunaScript SuperMix kit (NEB, E3010S) following the manufacturer's instructions. qPCR was performed in three technical replicates using SsoAdvanced Universal SYBR Green Supermix

(7) Neuronal hyperactivity becomes mTORC1 independent due to transcriptional changes in tuberous sclerosis complex disease modelsCell ReportsDecember 23, 2025Wardiya Afshar-Saber, Juan F. Ruiz, Isabel Gisser, Ziqin Yang, Leena Mehendale, Nicole A. Teaney, Rachel Hobson, Madison R. Glass, Truc T. Pham, Elizabeth Bainbridge, et al.Nanodrop (Themo Fisher), and cDNA was synthesized from RNA samples using LunaScript RT SuperMix Kit (E3010S; New England Biolabs). qPCR was performed using the Luna Universal qPCR Master Mix (M3003S; New England Biolabs

(8) Replicon-based genome-wide CRISPR knockout screening for the identification of host factors involved in viral replicationNat CommunDecember 10, 2025Karen W. Cheng, Madhura Bhave, Andrew L. Markhard, Duo Peng, Karan D. Bhatt, Katherine A. Travisano, Josette V. Medicielo, Astrid Anaya, Sanae Lembirik, Leila Njoya, et al.E3025S) was used to synthesize cDNA for EBOV vRNA, cRNA, and mRNA; a LunaScript RT supermix kit (NEB E3010S) was used to synthesize cDNA for the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA control.

(9) Excitatory cortical neurons from CDKL5 deficiency disorder patient-derived organoids show early hyperexcitability not identified in neurogenin2 induced neuronsNeurobiology of DiseaseOctober 15, 2025Madison R. Glass, Dosh Whye, Nickesha C. Anderson, Delaney Wood, Nina R. Makhortova, Taryn Polanco, Kristina H. Kim, Kathleen E. Donovan, Gayathri Rajaram Srinivasan, Lorenzo Vaccaro, et al.(Takara Bio catalog #740971) and converted into cDNA (LunaScript RT Supermix Kit New England Biolabs E3010S). Approximately 500 ng of cDNA was used per reaction (Luna Universal qPCR Master Mix New England Biolabs:

(10) Tumor cells upregulate CCL22 in a STING-dependent manner in response to paracrine factors released by STING-activated myeloid cells and type I interferonsImmunohorizonsOctober 8, 2025Elmira M Lomashvili, Jihyun Kim, Lingwei Kong, Pamela R Cookspectrophotometry. First-strand cDNA synthesis was performed using the LunaScript RT SuperMix Kit (NEB, catalog # E3010S) or LunaScript (-) RT SuperMix control with added M-MuLV Reverse Transcriptase (NEB). qPCR was performed

(11) An Integrative Genotyping and Gene Expression Profiling of the Mutated Human FAM111B Gene and Fibrosis-Associated Pathway in the POIKTMP SyndromeJ Cell Mol MedOctober 6, 2025Nadine Tambwe, Musalula Sinkala, Oluwafemi G. Oluwole, Nonhlanhla P. Khumalo, Afolake Arowolopurified total RNA was reverse transcribed (cDNA synthesis) using the LunaScript RT SuperMix kit (Cat. No. E3010S, New England BioLabs). The total RNA samples for the healthy control patients were pooled because they

(12) Ankyloblepharon–Ectodermal Defects–Cleft Lip/Palate Syndrome-Linked p63 Mutations Disrupt Keratinocyte Proliferation and Survival Through Oxidative Stress and Impaired Slc7a11 ExpressionInternational Journal of Molecular SciencesMay 29, 2025Daniela Di Girolamo, Sara Palumbo, Dario Antonini, Ludovica D’Auria, Vincenza Cerbone, Tommaso Porcelli, Federica Cavallo, Enzo Calautti, Chiara Riganti, Caterina Misseroprep kit (R2061, Zymo Research, Irvine, CA, USA). cDNA was synthesized using LunaScript RT-Supermix (E3010S, New England Biolabs, Ipswich, MA, USA). RT-qPCR to assess for mRNA relative expression performed using

(13) SARS-CoV-2 nsp1 mediates broad inhibition of translation in mammalsCell ReportsMay 27, 2025Risako Gen, Amin Addetia, Daniel Asarnow, Young-Jun Park, Joel Quispe, Matthew C. Chan, Jack T. Brown, Jimin Lee, Melody G. Campbell, Christopher P. Lapointe, et al.assays Quick-RNA Miniprep Kit,Zymo Research,R1054 LunaScript® RT SuperMix Kit,New England Biolabs,E3010S Zymoclean Gel DNA Recovery Kit,Zymo Research,D4002 KAPA HiFi HotStart ReadyMix,Roche,50-196-5299 CloneAmp™

(14) Coronary Artery Disease Risk Variant rs6903956 Links to Endothelial Dysfunction via PHACTR1 RegulationbioRxivMay 20, 2025Kai Yi Tay, Hannah Su-Ann Wee, Nhi Nguyen, Matias Ilmari Autio, Vanessa Kristina Wazny, Khang Leng Lee, Darwin Tay, Yuting Wang, Xu Gao, Chew Kiat Heng, et al.subsequently used to generate cDNA with LunaScript RT SuperMix Kit (New England Biolabs, catalog no. E3010S). Real-time PCR was performed using SYBR green gene expression assays (New England Biolabs, catalog

(15) A nucleosome switch primes hepatitis B virus infectionCellApril 17, 2025Nicholas A. Prescott, Tracy Biaco, Andrés Mansisidor, Yaron Bram, Justin Rendleman, Sarah C. Faulkner, Abigail A. Lemmon, Christine Lim, Rachel Tiersky, Eralda Salataj, et al.iTaq Universal SYBR Green Supermix,Bio-Rad,Cat# 1725121 Luna Universal qPCR Master Mix,NEB,Cat# M3003S LunaScript RT SuperMix Kit,NEB,Cat# E3010S Monarch RNA Cleanup Kit,NEB,Cat# T2040L NEBNext Ultra II DNA Library Prep Kit,NEB,Cat# E7645S Plasmid

(16) Adenine Base Editing with engineered Virus-Like Particles rescues the CFTR mutation G542X in patient-derived intestinal organoidsiScienceMarch 21, 2025Lucia Nicosia, Iwona Pranke, Roberta V. Latorre, Joss B. Murray, Lisa Lonetti, Kader Cavusoglu-Doran, Elise Dreano, James P. Costello, Michael Carroll, Paola Melotti, et al.any DNA carry-over. Extracted RNA was reverse transcribed using LunaScript RT SuperMix Kit (NEB, Cat#E3010S) according to the manufacturer’s protocols. qPCR was performed on sgRNA cDNA using the LightCycler 480_1536

(17) Quantitative expression of estrogen, progesterone and human epidermal growth factor receptor-2 and their correlation with immunohistochemistry in breast cancer at Uganda Cancer InstitutePLoS OneJanuary 3, 2025Henry Wannume, Nixon Niyonzima, Sam Kalungi, Julius Boniface Okuni, Tonny Okecha, Edward Kakungulu, Steven Mpungu Kiwuwa, Geoffrey Waiswa, Sylvester Kadhumbula, Monica Namayanja, et al.DNA (cDNA) synthesis Complementary DNA (cDNA) was synthesized using a LunaScript RT SuperMix Kit (E3010S) (New England BioLabs, Hitchin, England) according to the manufacturer’s instructions. A 20μL reaction

(18) OPG promotes lung metastasis by reducing CXCL10 production of monocyte-derived macrophages and decreasing NK cell recruitmentEBioMedicineJanuary 1, 2025Hu H, Li X, Xu Z, Tao Y, Zhao L, You H, Xu G, Zhang T, Zhang Y, Fan H, et al.Plus Micro Kit (QIAGEN #74034). Then, cDNA was synthesized using the LunaScript RT SuperMix Kit (NEB #E3010S), and the expression of the tested gene was subsequently analysed using THUNDERBIRD Next SYBR qPCR Mix

(19) The glycosyltransferase ST3GAL4 drives immune evasion in acute myeloid leukemia by synthesizing ligands for the glyco-immune checkpoint receptor Siglec-9LeukemiaNovember 17, 2024Vignesh Krishnamoorthy, John Daly, Jimmy Kim, Lidia Piatnitca, Katie A. Yuen, Bhoj Kumar, Mehrnoush Taherzadeh Ghahfarrokhi, Tom Q. T. Bui, Parastoo Azadi, Ly P. Vu, et al.RNA sample was synthesized using the protocol provided by the supplier (LunaScript® RT SuperMix Kit, E3010S, NEB), and then the experiment was set up using a pre-mixed qPCR master mix (Luna® Universal qPCR Master Mix

(20) Differentiation signals induce APOBEC3A expression via GRHL3 in squamous epithelia and squamous cell carcinomaThe EMBO JournalNovember 15, 2024Nicola J Smith, Ian Reddin, Paige Policelli, Sunwoo Oh, Nur Zainal, Emma Howes, Benjamin Jenkins, Ian Tracy, Mark Edmond, Benjamin Sharpe, et al.Monarch Total RNA Miniprep Kit,New England Biolabs,T2010S LunaScript® Reverse Transcriptase (RT) SuperMix,New England Biolabs,E3010S PowerUp™ SYBR™ Green Master Mix for qPCR,Thermo Fisher,A25742 Absolute Q™ DNA Digital PCR Master Mix

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LunaScriptRT SuperMix Kit

LunaScriptRT SuperMix Kit from New England Biolabs

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New England Biolabs

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LunaScriptRT SuperMix Kit from New England Biolabs

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