LunaScriptRT SuperMix Kit E3010L from New England Biolabs

Product Specs
ItemLunaScriptRT SuperMix Kit
CompanyNew England Biolabs
Price $470.00 Inquire
Catalog NumberE3010L
Quantity100 reactions
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Valproic acid remodels phosphatidylglycerol homeostasis and epithelial-macrophage crosstalk in inflammatory signalingiScienceSeptember 18, 2026Ivana Ďurišová, Mariana Máčajová, Ingrid Zriniová, Ivana Fábryová, Mailinda Meta, Lenka Bábelová, Boris Bilčík, Aleš Kvasnička, Miroslav Baláž, Soňa Bernátová, et al.K0732 Aurum Total RNA Mini Kit,Bio-Rad,Cat# 7326820 LunaScript RT SuperMix Kit,New England Biolabs,Cat# E3010L FastStart Essential DNA Green Master,Roche,Cat# 06402712001 Human IL-6 ELISA kit,PeproTech,Cat# 900-T16 Human

(2) Phosphoproteomic Insights into TRPV4-AMPK Signaling Axis and Barrier Regulation in the Choroid Plexus EpitheliumInt J Mol SciSeptember 18, 2026Gowthami Mahendran, Maryam Torabi, Bonnie L Blazer-YostcDNA synthesis. cDNA was synthesized from total RNA using LunaScript RT SuperMix Kit (NEB, Cat No: E3010L) and subsequently used for PCR analyses. Primer pairs designed using Primer-BLAST [64] and those obtained

(3) A tailored in vivo CRISPR screen identifies BAP1 as a potent tumor suppressor of sarcomaJCI InsightAugust 24, 2026Jianguo Huang, Xingliang Liu, Warren Floyd, William Haugh, Zhaoyu Sun, Melissa J. Kasiewicz, Yaping Wu, Brian Piening, John T. Welle, Wesley K. Rosales, et al.(PRJNA1212052). Real-time qPCR. cDNA was prepared using a LunaScript RT SuperMix kit purchased from New England Biolabs (E3010L). Quantitative reverse transcription PCR was performed using a QuantStudio 6 System (Applied Biosystems).

(4) Naturally acquired malaria is associated with shorter leukocyte relative telomere length and increased hTERT expression in an endemic nigerian populationFront Cell Infect MicrobiolAugust 6, 2026Theophilus Nang Wakai, Shalom Nwodo Chinedu, Israel Sunmola Afolabitranscription. Complementary DNA (cDNA) was synthesised from total RNA using LunaScript® RT SuperMix Kit (E3010L; New England Biolabs, USA), following the manufacturer’s protocol. The synthesised cDNA was used as

(5) Targeting CPSF73, the mRNA 3' end processing endonuclease, moves cancer cells away from the mesenchymal stateFront ImmunolAugust 5, 2026Marzieh Naseri, Huiyun Liu, Luyang Wang, Salwa Mohd Mostafa, Ehsan Ranaei Pirmardan, Bin Tian, Claire L. Moorefrom 1 μg of total RNA from each sample using LunaScript RT SuperMix Kit (New England BioLabs (NEB), E3010L). To perform RT-qPCR, Luna Universal qPCR Master Mix Kit (NEB, M3003L) and specific primers were used

(6) Chondroitin sulfate shapes the unfolded protein response in osteosarcoma to sustain cancer cell survival during ER stressCell Death & DiseaseAugust 1, 2026Maj Sofie Ørum-Madsen, Jessica Pihl, Thomas M. Clausen, Charlotte Spliid, Anne-Chloé Dhez, Hai-Feng Zhang, Yen-Hsi Chen, Elena Ethel Vidal-Calvo, Tobias Gustavsson, Michael Lizardo, et al.RNA was reverse-transcribed into cDNA using the LunaScript® RT SuperMix Kit (New England Biolabs, #E3010L) in a 20 µL reaction according to manufacturer’s protocol (reaction: 2 min at 25 °C, 10 min at 55 °C,

(7) Identification and functional characterization of regulatory variants in DPP9 associated with COVID-19 severityGenome MedicineJuly 2, 2026Gaëlle Farah, Magali Torres, Leo Henches, Hugues Aschard, Jade Ghosn, Xavier Duval, Milieu Intérieur Consortium, Laurent Abel, Andres Alcover, Philippe Bousso, et al.instructions. 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs, E3010L). qPCR reactions were made using SYBR green Master Mix (Thermo Fisher Scientific, 4,367,660) and measurement

(8) Single-Cell dissection of fibrodysplasia ossificans progressiva identifies SPP1 as a mediator of macrophage–fibroadipogenic progenitors crosstalkCellular and Molecular Life SciencesJuly 2, 2026Riccardo Gamberale, Mauro Bergamaschi, Anna Sofia Tascini, Cristina D’Orlando, Michela Signo, Antonello Spinelli, Raffaella Meneveri, Renata Bocciardi, Bénédicte Chazaud, Emanuele Azzoni, et al.samples were reverse transcribed to cDNA with the LunaScript RT SuperMix Kit (New England Biolabs, E3010L), using the Thermocycler 2027 (Applied Biosystems). Real-time rt-qPCR was carried out using the Luna Universal qPCR Master Mix

(9) Oncogenic KRAS-driven type I interferon signalling primes pancreatic cancer for necroptosisNat CommunJune 15, 2026Sofya Tishina, Alina Dahlhaus, Marta Manik, Lejla Mulalic, Janine Murr, Michael Kotliar, Hassan Rakhsh-Khorshid, Myrto Kostopoulou, Florian Hocher, Jenny Stroh, et al.manufacturer’s instructions. The isolated RNA was then converted to cDNA using the LunaScript RT SuperMix Kit (E3010L, New England Biolabs). For qPCR each reaction contained 5 µl of Luna Universal qPCR Master Mix (M3003E,

(10) Microbiota variation in Aedes aegypti (Diptera:Culicidae): a comparative study of field-caught and laboratory-adapted reared populationsMicrobiology SpectrumJune 2, 2026Abelgie Sánchez-Amézquita, David Martínez, Omar Cantillo-Barraza, Yurany Granada, Manuel Medina, Luz Helena Patiño, Marina Muñoz-Díaz, Juan David Ramírezextracted RNA via reverse transcription using the LunaScript RT SuperMix Kit (New England Biolabs, E3010L). The reaction was performed in a MiniAmp thermal cycler (Thermo Fisher Scientific) with the following

(11) An ancient retrotransposon provides species-specific tuning of IL-18 inflammatory signalingbioRxivMay 29, 2026Andrea D. Ordonez, Holly Allen, Lynn Sanford, Atma Ivancevic, Ashley Agyepong, Mia Chaw, James P. Bridges, Tony Schountz, Edward B. ChuongQuick-RNA Miniprep kit (Zymo Research; R1054) and reverse-transcribed with LunaScript RT SuperMix (NEB; E3010L) according to the manufacturers’ instructions. Mouse IL-18 pathway receptors and human IL18BP were amplified

(12) Single-cell transcriptomics highlights macrophage-driven regulation of EndMT and repair in injured muscleCommunications BiologyMay 11, 2026Filipa Timóteo-Ferreira, Mauro Bergamaschi, Riccardo Gamberale, Cristiana Barone, Cristina D’Orlando, Anna Sofia Tascini, Veronica Bonalume, Raffaella Meneveri, Alessandro Fantin, Emanuele Azzoni, et al.at 260 and 280 nm. RNA samples were reverse transcribed to cDNA with the LunaScript RT SuperMix Kit (E3010L) from New England Biolabs, using the Thermocycler 2027 (Applied Biosystems). Real-time rt-qPCR analysis Real-time

(13) Identification of the adhesion GPCR ADGRL4/ELTD1 as a novel potential prognostic biomarker for cerebral cavernous malformation diseaseMolecular MedicineMay 8, 2026Andrea Perrelli, Ashraqat Ahmed, Anna Onisiforou, Gwenola Boulday, Juan Zalvide, Celia M. Pombo, José E. Martínez, Alessio Paolini, Salim Abdelilah-Seyfried, Ioannis Petrakakis, et al.eluted in ultrapure H2O and immediately used to prepare cDNA using the LunaScript® RT SuperMix Kit (E3010L, New England BioLabs), and the DNA used for RT-PCR. RT-PCR was performed with 2 µl of 1 st cDNA strand

(14) Bi-allelic WDHD1 variants cause microcephalic primordial dwarfismThe American Journal of Human GeneticsMay 7, 2026Debora Tibbe, Marie Ronja Vogt, Tess Holling, Lea Dewi Schlieben, Fanny Kortüm, Moneef Shoukier, Christoph Bagowski, Felix Distelmaier, Luisa Averdunk, Alexej Knaus, et al.transcribed into complementary DNA (cDNA) using the LunaScript RT SuperMix Kit (New England Biolabs, #E3010L). Reverse-transcription PCR (RT-PCR) was performed using OneTaq Quick-Load 2× Master Mix (New England Biolabs

(15) Retrovirus insertions in host transcripts trigger de novo piRNA immunityThe EMBO JournalMay 2, 2026Baptiste Rafanel, Liudmila Protsenko, Dominik Handler, Julius Brennecke, Kirsten-André SentiScientific,MAN0012000 Zymo RCC-25 Kit,Zymo Research,R1017 LunaScript RT Supermix Kit,New England Biolabs (NEB),E3010L Luna Universal qPCR Master Mix,NEB,E3003L Monarch Genomic DNA Purification Kit,NEB,T3010L T4 RNA

(16) Pathogenic variants in the cohesin loader subunit MAU2 underlie a distinct Cornelia de Lange Syndrome subtypeNat CommunMarch 30, 2026Ilaria Parenti, Alina Hesters, Marta Gil-Salvador, Laura Duffy, Deniz Kanber, Jasmin Beygo, Jennifer Kerkhof, Laura Steenpaß, Elsa Leitão, Julia Woestefeld, et al.ReliaPrep™ RNA Cell Miniprep System (Promega) according to the manufacturer’s instructions. The LunaScript® RT SuperMix Kit (E3010L, New England Biolabs, Ipswich, MA, USA) was used to retro-transcribe 1 µg of RNA. cDNA synthesis was

(17) Dual roles of syndecan-4 in regulating chicken fibrosis in vitroFrontiers in PhysiologyMarch 23, 2026Lucie Pejšková, Nina Therese Solberg, Marianne Lunde, Cathrine Rein Carlson, Mona Elisabeth Pedersen, Sissel Beate Rønningwashed twice by cold PBS, and cDNA was generated from 300 ng or 1 µg using LunaScript RT SuperMix kit (#E3010L, New England BioLabs, Ipswich, MA, United States) in a 20 µL reaction volume with random hexamers, according

(18) Overexpression of MusaVicilin gene for disease resistance in bananaFrontiers in Plant ScienceMarch 23, 2026Sarah Wanjiku Macharia, Jaindra Nath Tripathi, Valentine Otang Ntui, Samwel Muiruri Kariuki, Leena TripathiRNA was converted to cDNA using the LunaScript® RT SuperMix cDNA Synthesis Kit (New England BioLabs, E3010L) as outlined in the user’s manual. The resulting cDNA was diluted ten-fold, and 5 μl was used as a template

(19) Immortalized Rat Tendon-Derived Stem Cells for Tendon Tissue EngineeringBioengineering (Basel, Switzerland)March 18, 2026Lau KT, Wang H, Zhang J, Wang DM, Ker DFE.transcription of 1 μg total RNA using LunaScript RT Supermix (New England Biolabs, Ipswich, MA, USA, Cat.: E3010L). qPCR was performed using a QuantStudio 7 (Flex) Real-time PCR System (Applied Biosystems, Waltham,

(20) Anabolic and catabolic responses to different modes of exercise in patients with chronic kidney diseaseBMC NephrologyMarch 13, 2026Douglas W. Gould, Luke A. Baker, Thomas J. Wilkinson, Nicholas Eastley, Robert U. Ashford, Matthew Denniff, Matthew Graham-Brown, João L. Viana, Andrew Philp, Emma L. Watsonand reversed transcribed to cDNA using Lunascript transcription system (New England BioLabs, MA, USA, E3010L). Primers and probes and internal controls were supplied as TaqMan gene expression assays (ThermoFisher,

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LunaScriptRT SuperMix Kit

LunaScriptRT SuperMix Kit from New England Biolabs

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New England Biolabs

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LunaScriptRT SuperMix Kit from New England Biolabs

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