LunaScriptRT SuperMix Kit E3010L from New England Biolabs

Product Specs
ItemLunaScriptRT SuperMix Kit
CompanyNew England Biolabs
Price $470.00 Inquire
Catalog NumberE3010L
Quantity100 reactions
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Oncogenic KRAS-driven type I interferon signalling primes pancreatic cancer for necroptosisNat CommunJune 15, 2026Sofya Tishina, Alina Dahlhaus, Marta Manik, Lejla Mulalic, Janine Murr, Michael Kotliar, Hassan Rakhsh-Khorshid, Myrto Kostopoulou, Florian Hocher, Jenny Stroh, et al.manufacturer’s instructions. The isolated RNA was then converted to cDNA using the LunaScript RT SuperMix Kit (E3010L, New England Biolabs). For qPCR each reaction contained 5 µl of Luna Universal qPCR Master Mix (M3003E,

(2) Microbiota variation in Aedes aegypti (Diptera:Culicidae): a comparative study of field-caught and laboratory-adapted reared populationsMicrobiology SpectrumJune 2, 2026Abelgie Sánchez-Amézquita, David Martínez, Omar Cantillo-Barraza, Yurany Granada, Manuel Medina, Luz Helena Patiño, Marina Muñoz-Díaz, Juan David Ramírezextracted RNA via reverse transcription using the LunaScript RT SuperMix Kit (New England Biolabs, E3010L). The reaction was performed in a MiniAmp thermal cycler (Thermo Fisher Scientific) with the following

(3) An ancient retrotransposon provides species-specific tuning of IL-18 inflammatory signalingbioRxivMay 29, 2026Andrea D. Ordonez, Holly Allen, Lynn Sanford, Atma Ivancevic, Ashley Agyepong, Mia Chaw, James P. Bridges, Tony Schountz, Edward B. ChuongQuick-RNA Miniprep kit (Zymo Research; R1054) and reverse-transcribed with LunaScript RT SuperMix (NEB; E3010L) according to the manufacturers’ instructions. Mouse IL-18 pathway receptors and human IL18BP were amplified

(4) Bi-allelic WDHD1 variants cause microcephalic primordial dwarfismThe American Journal of Human GeneticsMay 7, 2026Debora Tibbe, Marie Ronja Vogt, Tess Holling, Lea Dewi Schlieben, Fanny Kortüm, Moneef Shoukier, Christoph Bagowski, Felix Distelmaier, Luisa Averdunk, Alexej Knaus, et al.transcribed into complementary DNA (cDNA) using the LunaScript RT SuperMix Kit (New England Biolabs, #E3010L). Reverse-transcription PCR (RT-PCR) was performed using OneTaq Quick-Load 2× Master Mix (New England Biolabs

(5) Retrovirus insertions in host transcripts trigger de novo piRNA immunityThe EMBO JournalMay 2, 2026Baptiste Rafanel, Liudmila Protsenko, Dominik Handler, Julius Brennecke, Kirsten-André SentiScientific,MAN0012000 Zymo RCC-25 Kit,Zymo Research,R1017 LunaScript RT Supermix Kit,New England Biolabs (NEB),E3010L Luna Universal qPCR Master Mix,NEB,E3003L Monarch Genomic DNA Purification Kit,NEB,T3010L T4 RNA

(6) Pathogenic variants in the cohesin loader subunit MAU2 underlie a distinct Cornelia de Lange Syndrome subtypeNat CommunMarch 30, 2026Ilaria Parenti, Alina Hesters, Marta Gil-Salvador, Laura Duffy, Deniz Kanber, Jasmin Beygo, Jennifer Kerkhof, Laura Steenpaß, Elsa Leitão, Julia Woestefeld, et al.ReliaPrep™ RNA Cell Miniprep System (Promega) according to the manufacturer’s instructions. The LunaScript® RT SuperMix Kit (E3010L, New England Biolabs, Ipswich, MA, USA) was used to retro-transcribe 1 µg of RNA. cDNA synthesis was

(7) Overexpression of MusaVicilin gene for disease resistance in bananaFrontiers in Plant ScienceMarch 23, 2026Sarah Wanjiku Macharia, Jaindra Nath Tripathi, Valentine Otang Ntui, Samwel Muiruri Kariuki, Leena TripathiRNA was converted to cDNA using the LunaScript® RT SuperMix cDNA Synthesis Kit (New England BioLabs, E3010L) as outlined in the user’s manual. The resulting cDNA was diluted ten-fold, and 5 μl was used as a template

(8) Dual roles of syndecan-4 in regulating chicken fibrosis in vitroFrontiers in PhysiologyMarch 23, 2026Lucie Pejšková, Nina Therese Solberg, Marianne Lunde, Cathrine Rein Carlson, Mona Elisabeth Pedersen, Sissel Beate Rønningwashed twice by cold PBS, and cDNA was generated from 300 ng or 1 µg using LunaScript RT SuperMix kit (#E3010L, New England BioLabs, Ipswich, MA, United States) in a 20 µL reaction volume with random hexamers, according

(9) Immortalized Rat Tendon-Derived Stem Cells for Tendon Tissue EngineeringBioengineering (Basel)March 18, 2026Kat Tik Lau, Hui Wang, Jinxiang Zhang, Dan Michelle Wang, Dai Fei Elmer Kertranscription of 1 μg total RNA using LunaScript RT Supermix (New England Biolabs, Ipswich, MA, USA, Cat.: E3010L). qPCR was performed using a QuantStudio 7 (Flex) Real-time PCR System (Applied Biosystems, Waltham,

(10) Anabolic and catabolic responses to different modes of exercise in patients with chronic kidney diseaseBMC NephrologyMarch 13, 2026Douglas W. Gould, Luke A. Baker, Thomas J. Wilkinson, Nicholas Eastley, Robert U. Ashford, Matthew Denniff, Matthew Graham-Brown, João L. Viana, Andrew Philp, Emma L. Watsonand reversed transcribed to cDNA using Lunascript transcription system (New England BioLabs, MA, USA, E3010L). Primers and probes and internal controls were supplied as TaqMan gene expression assays (ThermoFisher,

(11) WWP1 gain-of-function drives developmental anoikis through TGFβ pathway during neurodevelopmentCell Death DiscoveryMarch 6, 2026Ki Hurn So, Seungbok Lee, Jiayi Wong, Hyunsik Lee, Eun-Jin Yun, Se Song Jang, Hee-Jung Choi, Jong-Hee Chae, Seung Tae Baeksequenced on the HiSeq2000 platform. cDNA was synthesized using LunaScript reverse transcriptase (NEB, #E3010L) or ImProm-II™ Reverse Transcriptase (Promega, #A3802). qPCR experiments were conducted with Luna Universal

(12) LPS-induced inflammation differentially affects endogenous Ca2⁺ activity in mouse and human iPSC-derived astrocytesMolecular MedicineMarch 5, 2026Franziska E. Müller, Flavian Ivanov, Anne-Catharine Studt, Ida Nitzsche, Frauke S. Bahr, Anna-Lena Krüger, Josephine Labus, Ghanendra Singh, Evgeni G. Ponimaskin, Kerstin Lenk, et al.Qiagen, Hilden, Germany). Subsequently, mRNA was transcribed to cDNA using LunaScript™ RT SuperMix Kit (#E3010L, NEB, Frankfurt, Germany). The cDNA was diluted 1:10 in H2O, and RTqPCR was performed using Luna® Universal Probe qPCR Master Mix

(13) Reversibility and therapeutic feasibility of DNM1L-associated neurodevelopmental disordersExperimental & Molecular MedicineMarch 5, 2026Ki Hurn So, Se Hee Kim, Shinyoung Jang, Hyeun Deok Sang, Eun-Jin Yun, Hee-Jung Choi, Jong-Hee Chae, Seung Tae Baekprep kit (Illumina) and sequenced by HiSeq2000. RNA was reverse transcribed with the LunaScript (NEB, #E3010L). The quantitative PCR (qPCR) analysis was performed using Luna Universal SYBR (NEB, #M3003L) on the

(14) E-cigarette exposure impairs skeletal muscle mitochondrial function in male micePhysiol RepMarch 4, 2026Pavel Mazirka, Jaewon Choi, Samuel Alvarez, Pascual Jahuey, Kerri A. O'Malley, Scott T. Robinson, Salvatore T. Scali, Terence E. Ryan, Scott A. Berceli, Kyoungrae Kiminstructions. cDNA was generated from RNA using the LunaScript RT Supermix kit (New England Biolabs, E3010L) according to the manufacturer's directions. Real‐time PCR (RT‐PCR) was performed on a Quantstudio 3

(15) Induction of collective behavior by β-1,3-glucans in microalgaeScience AdvancesJanuary 30, 2026Lou Lambert, Richard G. Dorrell, Antoine DanonQuick-RNA MiniPrep Kit (ZR1054, Zymo Research), and RT was performed using the LunaScript RT Supermix Kit (E3010L, New England Biolabs). Detection and quantification of cell wall sugars β-1,3-glucans were detected

(16) The effects of hypoxia inducible factor-1α in myeloid cells and a high-salt diet in a mouse model of orthodontic tooth movementKorean Journal of OrthodonticsJanuary 25, 2026Valentin Jüngel, Eva Paddenberg-Schubert, Kathrin Renner, Anna Damanaki, Tobias Bopp, Christian Kirschneck, Agnes Schröder, Jonathan Jantsch, Peter Proffof RNA were prepared in a final volume of 4 µL and mixed with 1 µL of Luna Script RT SuperMix Kit (E3010L; New England Biolabs, Frankfurt am Main, Germany). Conversion into cDNA was performed in Thermocycler

(17) NSD2 upregulation is driven by high-risk HPV E6/E7 and disrupts epithelial differentiation in HPV-associated head and neck cancerJournal of Experimental & Clinical Cancer ResearchJanuary 8, 2026Lavinia Ghiani, Simona Citro, Alessandro Medda, Mirko Doni, Farkhondeh Ghoryani, Roberta Noberini, Ottavio Croci, Fausto Maffini, Claudia Miccolo, Laura Monteleone, et al.generated by reverse transcription-PCR with with the LunaScript™ RT SuperMix Kit (New England Biolabs #E3010L). Relative levels of specific mRNAs were determined with Luna Universal qPCR master mix (New England Biolabs

(18) Mammalian metaphase kinetochores are elastic and require condensin for robust structure and functionBIORXIVDecember 25, 2025Vanna M. Tran, Jinghui Tao, Caleb J. Rux, David Gomez Siu, Miquel Rosas-Salvans, Sophie Dumont2015). They were then verified with gel electrophoresis using the Lunascript Kit (New England Biolabs; E3010L) to generate PtK2 cDNA from purified total RNA, and then performing PCR to check that the amplicon is

(19) Lack of caspase 8 directs neuronal progenitor-like reprogramming and small cell lung cancer progressionNat CommunDecember 18, 2025Ariadne Androulidaki, Fanyu Liu, Christina M. Bebber, Ilmars Kisis, Vignesh Sakthivelu, Pascal Hunold, Lioba Koerner, Alina Dahlhaus, Fatma Isil Yapici, Christina Grimm, et al.protocol. The isolated RNA was reverse transcribed into cDNA using the LunaScript RT SuperMix Kit (E3010L, NEB) following the protocol provided by the manufacturer. For quantitative PCR, 5 μl of Power SYBR Green PCR Master Mix

(20) RIPK1 autophosphorylation at S161 mediates cell death and inflammationThe Journal of experimental medicineDecember 1, 2025Koerner L, Li X, Silnov E, Laurien L, Pasparakis M.extracted using a Direct-zol RNA isolation kit (Biozol). cDNA was prepared using the LUNA RT SuperMix kit (E3010L; NEB). All qRT-PCRs were performed with TaqMan probes (Il1b Mm00434228_m1, Il6 Mm00446190_m1, Tnf Mm00443258_m1,

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LunaScriptRT SuperMix Kit

LunaScriptRT SuperMix Kit from New England Biolabs

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New England Biolabs

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LunaScriptRT SuperMix Kit from New England Biolabs

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