Fig 1: The docking model of isorhamnetin, quercetin, calycosin, formononetin, and kaempferol with ESR1. (A) The network diagram of 10 hub genes and 5 HQ active ingredients was constructed. (B) Molecular docking map of 5 HQ active ingredients and ESR1.
Fig 2: Effects of five active compounds on ESR1 level in Ang II‐induced cardiomyocytes. Cardiomyocytes were treated with Ang II (1 μM) for 24 h in the presence or absence of Quercetin (20 μM), Isorhamnetin (50 μM), Calycosin (20 μM), Kaempferol (20 μM), or Formononetin (20 μM) and then examined for the ESR1 level in cardiomyocytes by ELISA assay (A); the protein levels of ESR1 and phosphorylated‐ESR1 in cardiomyocytes by western blot assay (B). N = 3 (biological replicates). ***p < 0.001 compared to control group; # p < 0.05, ## p < 0.01, ### p < 0.001 compared to Ang II group.
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