Fig 1: AhR agonist activity in healthy plasma samples with insulin sensitivity markers was assessed in technical duplicates using an AhR ligand sensitive luciferase assay. AhR activity levels of healthy participants were positively correlated with FBG (A), insulin (B), HbA1C (C), and HOMA‐IR (D). Values represent the actual measurements of each sample. Correlations between Ahr activity levels and metabolic markers were assessed using Spearman's rank correlation coefficient, p < 0.05 was considered significant.
Fig 2: AhR agonist activity in healthy plasma samples was assessed in technical duplicates using an AhR ligand sensitive luciferase assay. AhR activity levels of healthy participants were positively correlated with IL‐1β (A), IL‐6 (B), TNF‐α (C) TNF‐β (D), MCP1 (E). Values represent the actual measurements of each sample. Significance levels were determined using comparisons between the two groups using the Mann–Whitney U test. For comparisons among three or more groups, the Kruskal–Wallis test followed by Dunn's corrected post hoc multiple comparisons test was applied. Correlations between Ahr activity/protein levels and metabolic and inflammatory markers were assessed using Spearman's rank correlation coefficient. p‐value < 0.05 was considered statistically significant.
Fig 3: AhR protein levels and AhR agonist activity in normal/healthy weight, overweight and obese plasma samples. (A): AhR plasma protein levels of healthy normal/healthy weight, overweight and obese were assessed in triplicate (n = 3) using Human Aryl Hydrocarbon Receptor ELISA Kit. (B): AhR agonist activity in plasma samples of normal/healthy weight (n = 18), overweight (n = 23) and obese (n = 39), was assessed in technical duplicates using an AhR ligand sensitive luciferase assay. (C): Solid line shows linear regression with correlation of AhR activity and AhR soluble protein in plasma. Values represent the actual measurements of each sample. Lines represent mean and error bar SEM. For comparisons among three or more groups, the Kruskal–Wallis test followed by Dunn's corrected post hoc multiple comparison test was applied. p < 0.05 was considered significant.
Fig 4: AhR agonist activity in healthy plasma samples with clinical markers was assessed in technical duplicates using an AhR ligand sensitive luciferase assay. AhR activity levels of healthy participants were positively correlated with weight (A), waist circumference (B), BMI (C), TG (D), Cholesterol (E), and HDL (F). Values represent the actual measurements of each sample. Correlations between Ahr activity levels and metabolic markers were assessed using Spearman's rank correlation coefficient, p < 0.05 was considered significant.
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