Fig 1: Incomplete activation of dendritic cells (DCs) by CFA. The gating strategy for analysing the DC subset and macrophages by flow cytometry is shown in Figure S10. (a) The cell number and frequency of APCs in spleen were analysed. (b) The presentation functionality of cDC2 and macrophage in spleen were characterized by the expression of MHC‐II. (c) Cell populations were overlapped in histogram for comparison of the capacity of IL‐10 production of major innate and adaptive immune cells. (d) Flow cytometry analysis of the cell number and frequency of IL10+ neutrophils in the spleen. The results are representative of two independent experiments and shown as mean ± SD. Statistical significance between groups is indicated by *p < .05, **p < .01 and ***p < .001. DC, dendritic cell; cDC1, conventional DC 1; cDC2, conventional DC 2; MHC‐II, major histocompatibility complex class II molecule; pDC, plasmacytoid DC.
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