Fig 1: Quantification of WBCs, EOSs, IL-5, IL-33 and IFN-γ in the bronchoalveolar lavage fluid of rats from four different groups. Quantification of (A) WBCs and (B) EOSs in each group. ELISA results showing the levels of (C) IL-5, (D) IL-33 and (E) IFN-γ in each group. **P<0.01 vs. the control group; ##P<0.01 vs. the siRNA group. WBCs, white blood cells; EOSs, eosinophils; siRNA, small interfering RNA.
Fig 2: (a) The oxidative stress markers of groups. A: p < 0.05 for CIN + DRs versus HRs, and DRs regarding LPO and MPX levels. B: p < 0.05 for DRs versus HRs, regarding LPO and MPX levels. C: p < 0.05 for HRs versus MTDRs and MTCIN + DRs, regarding LPO and MPX levels. D: p > 0.05 for MTDRs versus MTCIN + DRs regarding LPO and MPX levels. E: p < 0.05 for MTCIN + DRs versus MTDRs regarding MPX levels. (b) The kidney tissue levels of IL-33, IL-6, and IL-1β of groups. A: p < 0.05 for CIN + DRs versus HRs, and DRs regarding IL-1β and IL-33 levels. B: p < 0.05 for DRs versus HRs, regarding IL-1β, IL-6, and IL-33 levels. C: p < 0.05 for HRs versus MTDRs and MTCIN + DRs, regarding IL-1β, IL-6, and IL-33 levels. D: p < 0.05 for MTDRs versus MTCIN + DRs regarding IL-6 and IL-33 levels. E: p < 0.05 for MTCIN + DRs versus CIN + DRs regarding IL-6 and IL-33 levels.
Fig 3: Correlation analysis of EOSs, IL-5, IL-33, IFN-γ and Src protein with Src mRNA in asthma model rats treated with Src small interfering RNA. Correlation of Src mRNA expression with (A) EOSs, (B) IL-5, IL-33, (C) IFN-γ and (D) Src protein. EOSs, eosinophils; siRNA, small interfering RNA.
Fig 4: Relative mRNA expression of inflammatory genes and levels of cytokines in the lung tissue of rats in each group. (A–D) Relative mRNA expressions of Il6, Il1ra, Il1b, and Plaur of lung tissue in the different groups of rats. (E–L) The levels of IL‐6, IL‐1α, IL‐1β, IL‐12p70, IL‐33, IL‐18, TNF‐α, and KC in lung tissue homogenate of rats in three groups were examined. Six rats per group, and data from each group were repeated three times in each experiment. The data were pooled together for analysis. Room air group (n = 3–6), hyperoxia group (n = 4–10), hyperoxia with GB treatment group (n = 5–13).
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