Fig 2: FKN deficiency suppressed F4/80, iNOS and ARG‐1 protein localization in mice kidney tissues via inhibition of Wnt/β‐catenin signalling. Scale bars represent 50 μm

Fig 3: FKN regulated polarization in LPS‐induced J774A.1 cells via Wnt/β‐catenin signalling. A, B, Western blot analysis and their respective quantitation were performed to detect the expression levels of iNOS, TNF‐α, ARG‐1 and IL‐10 protein in J774A.1 cells. C, D, ELISA was used to detect the levels of TNF‐α and ARG‐1 in the cell supernatants. * P < .05 compared with the control group; # P < .05 compared with the LPS group. E, F, Immunofluorescence analysis was used to ascertained the subcellular localization of iNOS and ARG‐1. Scale bars represent 10 μm

Fig 4: FKN deficiency suppressed macrophages polarization and proliferation in mice kidney tissues via inhibition of Wnt/β‐catenin signalling. A, B, Western blot analysis and their respective quantitation showing the protein expression of FKN, β‐catenin, Wnt‐4, c‐myc, cyclinD1, iNOS, TNF‐α, ARG‐1 and IL‐10 in mice kidney tissues. * P < .05 compared with the WT mice; # P < .05 compared with the LPS mice

Fig 5: M1/M2-associated gene expression changes after JWH133, GP1a or AM630 treatment. a-d M1-associated mRNA expressions, including IL-6, IL-12, CD86 and iNOS. e-h M2-associated mRNA expressions, including IL-4, IL-10, CD206 and Arg-1. Data are mean ± SD. + P < 0.05, ++ P < 0.01, JWH133 versus vehicle; * P < 0.05, ** P < 0.01, GP1a versus vehicle; # P < 0.05, ## P < 0.01, AM630 versus vehicle