Fig 1: Cytokine results. Panels A, C, and E are the standard curves for the IL-6, IL-8, and LITAF ELISA assays, respectively. Panels B, D, and F present the blood concentrations of IL-6, IL-8, and LITAF, calculated from the absorbance reading of each sample compared to the standard curve. * P < 0.05. ** P < 0.01. Abbreviations: HD-IQA, high dose IQA, DAE6; LD-IQA, low dose IQA; NC, negative control, DAE6; n.s., not significant; PC, positive control.
Fig 2: Effect of the RORα/RORγ/P65 on the Bcl-2/Bax and TNF-α/IL-6 expression. (A) Protein levels of Bcl-2; (B) protein levels of Bax; (C) TNF-α concentrations; (D) IL-6 concentrations. SR3335: RORα antagonist (5 μM); SR1078: RORα and RORγ agonist (10 μM); BAY: P65 antagonist (1 μM); ConA: 20 µg/mL, Melatonin: 10−9M. Data are presented as the means ± SEM. Different letters on the column indicate significant differences compared with the control group (P < 0.05).
Fig 3: Effect of monochromatic light on the concentrations of inflammatory factor in the thymus of chicks. (A) INF-γ concentrations; (B) IL-6 concentrations; (C) TNF-α concentrations. Data are presented as the means ± SEM. Different letters on the column indicate significant differences (P < 0.05) between treatment groups. * represents a significant difference in the pinealectomy group (P < 0.05) compared with the corresponding intact and sham operations. Abbreviations: BL, blue light; GL, green light; RL, red light; WL, white light.
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