Fig 1: Cell plasticity of MDA-MB-231 cells precultured in different cell culture configurations. (a) WB corresponding to the intracellular STC1 expression of “primed” MDA-MB-231 cells further cultured for 2 days on TCP under the same cell densities. a-Tubulin was used as a loading control. (b) Bar graph corresponding to secreted STC1 normalized to cell number. STC1 is measured by ELISA from the supernatant of “primed” MDA-MB-231 cells after 2 days of culture on TCP under the same cell densities and normalized to the cell number shown in the (e) bar graph. (c) Bar graph corresponding to STC2 secretion normalized to cell number. STC2 is measured by ELISA from the supernatant of “primed” MDA-MB-231 cells after 2 days of culture on TCP under the same cell densities and normalized to the cell number shown in the (f) bar graph. (d) Bar graph representing WB semiquantitative analysis of STC1 expression corresponding to STC1 expression after 2 days of cell culture and normalized to a-tubulin. (e) Average cell number determined by DNA quantification corresponding to each culture condition of STC1 experiments. (f) Average cell number determined by DNA quantification corresponding to each culture condition of STC2 experiments. Biological triplicates were used per experiment, and technical triplicates were used during the performance of ELISAs. Error bars represent SD. One-way ANOVA shows no statistical differences among all groups of “primed” cells once cultured for 2 additional days on 2D TCP. One-way ANOVA shows no statistical differences of cell number among all groups in the bar graph (e) corresponding to STC1 secretion experiments and the bar graph (f) corresponding to STC2 secretion experiments.