Fig 1: Two proteins were finally filtered for distinguishing mild/severe steatosis. (A) Among 12 clusters, cluster 3 included consistently up-regulated proteins from healthy controls to mild hepatic steatosis and to severe hepatic steatosis patients, whereas clusters 1 and 8 included consistently down-regulated proteins. (B) The GO analysis and expression (log2) of the filtered proteins (red color for up-regulated and green for down-regulated). (C) The Pearson association of 9 unique filtered proteins with clinical parameters. (D) The expression intensity (Un-log2) of selected ORM1 and ceruloplasmin, which were most strongly correlated with liver fat content (measured by MRI-PDFF), fibrosis, ALT, triglyceride, glucose, HOMA-IR, and C-reactive protein. (E) The diagnostic accuracy of ORM1 and ceruloplasmin for hepatic steatosis from healthy controls (right panel) and severe hepatic steatosis from mild hepatic steatosis patients (right panel) was calculated based on the expression intensity of proteomic data.