Fig 1: Evaluation of biomarker diagnostic accuracy for CRC. (A) ROC plots of mSEPT9, IGFBP2, DKK3, and PKM2 analyzed individually for CRC detection in the overall cohort (left panel) or stratified by tumor stage: early-stage disease (middle panel) or advanced-stage disease (right panel). Red = mSEPT9, blue = IGFBP2, green = DKK3, and gold = PKM2. (B) ROC plots of mSEPT9, IGFBP2, DKK3 and PKM2 analyzed with multivariate models for CRC detection in the overall cohort (left panel), early-stage disease (middle panel), or advanced-stage disease (right panel). Analyses included all four putative biomarkers (Model 1, red), mSEPT9 + IGFBP2 + DKK3 (Model 2, blue), and mSEPT9 + IGFBP2 (Model 3, green).