Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Total Procollagen Type I Intact N-terminal Propeptide (also known as total-P1NP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat total-P1NP antibody. total-P1NP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat total-P1NP Antibody is added and binds to total-P1NP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated total-P1NP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat total-P1NP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm