Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Angiotensin I (also known as ANG-I) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ANG-I antibody. ANG-I present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ANG-I Antibody is added and binds to ANG-I in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ANG-I antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ANG-I. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm