Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Sheep Toll-like Receptor 4 (also known as TLR4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Sheep TLR4 antibody. TLR4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Sheep TLR4 Antibody is added and binds to TLR4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TLR4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Sheep TLR4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm