GSH+GSSG / GSH Assay Kit (Colorimetric) ab239709 from Abcam

Product Specs
ItemGSH+GSSG / GSH Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab239709
Quantity100 Test, 2000 Test
Research AreaMetabolism

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) N-Acetylcysteine protects the developing brain in neonatal sepsis-like inflammation via a redox–neurovascular pathwayJournal of NeuroinflammationJune 30, 2026Ping Cheng, Shan Zhang, Xiaoli Zhang, Wenhua Li, Mengmeng Du, Mingchao Li, Zengyuan Yu, Jinjin Zhu, Guixiang Zeng, Yiran Xu, et al.(GSH), malondialdehyde (MDA), and 3-nitrotyrosine (3-NT) using commercially available assay kits (Abcam; ab239709, ab118970, and ab116691, respectively) according to the manufacturers’ instructions. Values were normalized

(2) Quantitative dissection of radioresistance in cancer cells: Oxygen depletion-induced biological versus chemical mechanisms and baseline mechanisms governed by HIF-1β-NRF2/TXNRD1NeoplasiaMay 15, 2026Satoshi Takeuchi, Minoru Kobayashi, Peter Wai Tik Lee, Itsuki Takahashi, Toshitada Yoshihara, Hiroshi Haradareduced glutathione (GSH) levels were quantified using the Glutathione Colorimetric Assay Kit (Abcam, Cat#ab239709), according to the manufacturer’s instructions. DCFH-DA assay Cells were seeded into 24-well plate

(3) Dynamics and reversibility of hepatic steatosis in male Mule duckFront PhysiolApril 16, 2026Ophélie Bernardi, Maxime Reverchon, Christelle Ramé, Eleni Zoi Glaveli, Hervé Rémignon, Pascal Froment, Joëlle Dupontoxidized glutathione (GSH/GSSG ratio) was measured following the manufacturer’s protocol (Cat. No. ab239709, Abcam, Cambridge, UK). Results are expressed in µmol/mg of protein. The activities of superoxide dismutase

(4) A self-supplying H2O2 “nano-reaction engine” with photothermal boosting for cascade chemodynamic–immunotherapyMaterials Today BioApril 1, 2026Beibei Sun, Tingsong Zhang, Ruoxi Wan, Leijie Tong, Tao Li, Jinyan Wang, Runwei Wang, Fei Yan, Shilun Qiucentrifuged, and the GSH content in the supernatants was measured using a GSH + GSSG/GSH Assay Kit (Abcam, ab239709) according to the instructions. The assay is based on the specific reaction between the thiol (-SH)

(5) Flavin adenine dinucleotide increases antioxidant availability and protects neonatal C57Bl6 lungs from high oxygen induced lung injuryAmerican Journal of Physiology-Cell PhysiologyApril 1, 2026Hunter D. Montgomery, Mingyang A. Zhang, Elizabeth Zimmerman, My N. Helmsoxidative stress and can be measured using a commercially available assay (GSH Assay Kit, catalog #ab239709, Abcam, Cambridge, MA). Manufacturer protocol for measuring GSH/GSSG Eh was followed without deviation.

(6) DGAT1 Inhibition Induces Ferroptosis and Enhances Cancer Immunotherapy EfficacyCancer ResMarch 11, 2026Dong Pan, Meng Jiao, Yanyan Zhu, Mengjie Hu, Fang Li, Jinming Yu, Chuan-Yuan Liassay Intracellular glutathione (GSH) levels were measured using the GSH+GSSG/GSH Assay Kit (Abcam, cat. #ab239709). Following the manufacturer’s instructions, cells were collected by centrifugation and lysed in ice-cold

(7) Disrupted energy metabolism is associated with retinal ganglion cell degeneration in autosomal dominant optic atrophyScience AdvancesFebruary 20, 2026Eugene Yu-Chuan Kang, Yun-Ju Tseng, Wei-Hao Peng, Hui-Chuan Hung, Pei-Hsuan Lin, Katrina P. Montales, Emmet Sherman, John Peregrin, Ethan Hunghsi Wang, Chunya Kang, et al.from mouse retinas Total GSH and reduced GSH levels were measured using a commercially available kit [ab239709, GSH+GSSG/GSH Assay Kit (Colorimetric), Abcam], following the manufacturer’s instructions. Retinal tissues

(8) Redox-active nitroxides enhance cisplatin efficacy against cervical cancerRedox BiologyFebruary 1, 2026Carl P. Soltau, Debottam Sinha, Lakshita P. Patil, Philip M. Moseley, Cassie L. Rayner, Nigel L. Barnett, Derek J. Richard, Steven E. Bottle, Ian H. Frazer, Alexander P. Martynglutathione dependent peroxidases was quantified using the colourimetric GSH + GSSG/GSH Assay Kit (Abcam, Cat#ab239709) and Glutathione Peroxidase Assay Kit (Abcam, Cat#ab102530) as per manufacturer's instructions. 18

(9) Trophoblast ferroptosis restricts SARS-CoV-2 spread in the placentabioRxivJanuary 27, 2026Eliza R McColl, Deepak U Kumar, Brittany R Jones, Nikole Marks, Emily Diveley, Rowan Karvas, Thorold W Theunissen, Jeannie C Kelly, Indira U Mysorekarfrozen placental villous tissue were quantified using a colorimetric GSH+GSSG/GSH Assay Kit (Abcam, ab239709). Approximately 100 mg of tissue was homogenized in Glutathione Reaction Buffer followed by addition

(10) Diabetes Induces Accumulation of Carbonylated Proteins in the Rat Retinal Pigment Epithelium Independently of Oxidative StressFASEB BioAdvancesJanuary 21, 2026Elena Morales‐Ramírez, Juan David Villeda‐González, Gustavo Sánchez‐Chávez, Rocío Salcedachemicals and DC Protein Assay kit were purchased from Bio‐Rad Laboratories; glutathione Kit (Abcam ab239709); quercetin, L‐NAME (Nω‐nitro‐L‐arginine methyl ester hydrochloride N‐5751), nitroblue tetrazolium chloride

(11) PLAGL1 overexpression exacerbates type 1 diabetes by inducing β-cell apoptosis via oxidative stress-dependent dual DNA damage and cGAS/STING pathway activationJournal of diabetes investigationJanuary 1, 2026Li C, Qiao L, Ge J, Hu S, Yang H, Hu C, Li T.Superoxide Dismutase (SOD) activity assay kit (Cat. No. ab65354) and GSH/GSSH detection kit (Cat. No. ab239709) were both purchased from Abcam (UK). The OxiSelect single‐cell gel electrophoresis kit (Cat. No. STA‐355‐5)

(12) Ataxia-telangiectasia mutated activation mediates transforming growth factor beta signaling in acetaminophen-induced liver injury in micePhysiol RepDecember 9, 2025Matthew McMillin, Christopher S. Chu, Elaina Williams, Juliet Venter, Kiersten Bell, Anca D. Petrescu, Patrick Mireles, Sharon DeMorrowLaboratories, Inc. (Houston, TX). Total liver GSH levels were measured using a GSH Assay Kit (Cat# ab239709) from Abcam (Cambridge, United Kingdom). The total input for the GSH Assay Kit was 50 mg of liver tissue

(13) HDGF Protects Retinal Pigment Epithelium from Glyoxal-Induced Ferroptosis via SIRT1/PGC-1α/Nrf2 PathwayAntioxidants (Basel)November 28, 2025Heng-Dao Lin, Rong-Kung Tsai, Yao-Tseng Wen, Pei-Kang Liureduced glutathione (GSH) levels were measured using the Total Glutathione Colorimetric Assay Kit (ab239709; Abcam; Waltham, MA, USA). Following treatment, cells were washed three times with PBS and harvested

(14) Polydatin Prevents UVA-Induced Damage in Human Dermal Fibroblasts by Maintaining Mitochondrial IntegrityCellsOctober 30, 2025Benedetta Niccolini, Alessia Riente, Duaa Hatem, Patrizia Bottoni, Michela Pizzoferrato, Giuseppe Tringali, Elisabetta Tabolacci, Giuseppe Maulucci, Stefano Marini, Chiara Ciaccio, et al.Total and Reduced Glutathione Assay Glutathione levels were measured using a colorimetric assay kit (ab239709, Abcam, Cambridge, UK) to determine total (GSH + GSSG) and reduced glutathione (GSH). Fibroblasts seeded

(15) Fungal mitochondria govern both gliotoxin biosynthesis and self-protectionmBioOctober 8, 2025Patrícia Alves de Castro, Endrews Delbaje, Ivan Lucas de de Freitas Migliorini, Monica T. Pupo, Muhammad Shafiul Alam Mondal, Karin Steffen, Antonis Rokas, Stephen K. Dolan, Gustavo H. Goldmanconcentrations Total glutathione levels were determined with the GSH + GSSG/GSH Assay Kit (Colorimetric) (Abcam, ab239709). Mycelia were ground to a fine powder in liquid nitrogen and re-suspended in Glutathione buffer (provided

(16) PNKP targeting engages the autophagic machinery through STING and STAT3 to potentiate ferroptosis and chemotherapy in TNBCRedox BiologyOctober 1, 2025Avi Maimon, Pier Giorgio Puzzovio, Yaron Vinik, Gavriel-David Hannuna, Sara Donzelli, Daniela Rutigliano, Giovanni Blandino, Sima Levcontrol cells. 4.7 GSH measurement GSH levels were measured by the Glutathione Colorimetric Assay kit (ab239709, Abcam, Cambridge, UK) according to the manufacturer's instructions and as previously described [21]. 4.8

(17) Chronic Pod-Mod E-Cigarette Aerosol Exposure Induces Aortic Dysfunction in Hypercholesterolemic Mice: Role of Oxidative Stress and InflammationArteriosclerosis, Thrombosis, and Vascular BiologySeptember 1, 2025Yasmeen M. Farra, Simone Sabnis, Jacqueline Matz, Hannah I.B. Wilker, Victoria A. Williams, Oliver Trejo, Hannah Kim, Cristobal F. Rivera, John Vlahos, Bhama Ramkhelawon, et al.the ratio of reduced (GSH) to oxidized (GSSG) circulating glutathione with a commercial ELISA kit (ab239709, Abcam; Cambridge, UK). Precautions were taken to protect against GSH (auto)oxidation by collecting

(18) Proteomic analysis reveals inhibition of mevalonate and glycolysis pathways in hepatocytes by 27-hydroxycholesterolBiochemical JournalAugust 6, 2025Wan-Seog Shim, Seulah Lee, Bakhovuddin Azamov, Chanhee Lee, Yeowon Kang, Kwang Min Lee, Changwan Hong, Sang-Mo Kwon, Koanhoi Kim, Dongjun Lee, et al.and GSH:GSSG ratio levels were measured using the GSH+GSSG/GSH colorimetric assay kit (Colorimetric) (ab239709, Abcam, Cambridge, U.K) following the manufacturer’s instructions. The absorbance was measured at 405

(19) Proteomic analysis reveals inhibition of mevalonate and glycolysis pathways in hepatocytes by 27-hydroxycholesterolBiochem JAugust 4, 2025Wan-Seog Shim, Seulah Lee, Bakhovuddin Azamov, Chanhee Lee, Yeowon Kang, Kwang Min Lee, Changwan Hong, Sang-Mo Kwon, Koanhoi Kim, Dongjun Lee, et al.and GSH:GSSG ratio levels were measured using the GSH+GSSG/GSH colorimetric assay kit (Colorimetric) (ab239709, Abcam, Cambridge, U.K) following the manufacturer’s instructions. The absorbance was measured at 405

(20) Chronic Pod-Mod E-Cigarette Aerosol Exposure Induces Aortic Dysfunction in Hypercholesterolemic Mice: Role of Oxidative Stress and InflammationArterioscler Thromb Vasc BiolJuly 24, 2025Yasmeen M. Farra, Simone Sabnis, Jacqueline Matz, Hannah Wilker, Victoria A. Williams, Oliver Trejo, Hannah Kim, Cristobal Rivera, John Vlahos, Bhama Ramkhelawon, et al.the ratio of reduced (GSH) to oxidized (GSSG) circulating glutathione with a commercial ELISA kit (ab239709, Abcam; Cambridge, UK). Precautions were taken to protect against GSH (auto)oxidation by collecting

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GSH+GSSG / GSH Assay Kit (Colorimetric) from Abcam

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GSH+GSSG / GSH Assay Kit (Colorimetric) from Abcam

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