Fig 1: Effects of hispolon on pro-inflammatory and anti-inflammatory cytokine levels, growth factor concentrations, and collagen properties in granulation tissue of dead-space wounds in rats after 7 days. (A) The levels of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) and the anti-inflammatory cytokine IL-10 were quantified. (B) The concentrations of growth factors, including FGF21, TGF-β1, EGF, and VEGF, were measured. (C) The properties of collagen, focusing on Type I and Type III collagen, were analyzed. Values (mean ± SD) were obtained from each group of 8 animals. a p < 0.05 and b p < 0.01, compared with the values of normal rats without treatment. c p < 0.05 and d p < 0.01, compared with the values of STZ-diabetic rats without treatment.
Fig 2: Effect of Orz on the scratch closure in vitro. (A)The cells were observed to migrate towards the wound gap after 12 and 24 h of incubation, as seen in the representative optical images (left panel). The scale bar indicates 200 μm. The scratch closure area was then quantified using Image J 1.38 software (right panel). (B) The changes of FGF21 and TGF-β1 in the culture supernatant were measured after 12 h of the scratch assay. The data were expressed as the mean ± SD of five independent experiments (n = 5) performed in triplicate. ap < 0.05 and bp < 0.01 compared to the untreated control group (control).
Supplier Page from Abcam for Rat FGF-21 ELISA Kit