Fig 1: Effect of HY7302 on serum and corneal tissue pro-inflammatory factor levels in BAC-induced DE mice. (A) Western blot images of extracellular signal-regulated kinase (ERK), phospho-ERK (p-ERK), c-Jun N-terminal kinase (JNK), phospho-JNK (p-JNK), matrix metalloproteinase-9 (MMP-9), inteleukin-1 beta (IL-1β), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (B) Quantification of phosphoprotein ratios or protein levels relative to CON. Serum concentrations of (C) matrix metalloproteinase-9 (MMP-9) and (D) interleukin-20 (IL-20) were measured using ELISA. Data are expressed as the mean ± SD (n = 6). Values with different letters are significantly different; p < 0.05 (a > b > c > d).
Fig 2: Effect of HY7302 on gut microbiota diversity, Spearman’s correlation, and relative species-level abundances in BAC-induced DE mice. (A) Box plots of microbial alpha diversity of each group was calculated using observed features. (B) PCoA plots of the bacterial community using unweighted UniFrac distance and PERMANOVA were used to measure the dissimilarity between groups. (C) Spearman’s correlation analysis between genus-level taxonomy profiles and DE indicators (serum MMP-9, serum IL-20, TV, TBUT, and corneal epithelial detachment). Regarding the heatmap, red squares indicate positive correlations and blue squares indicate negative correlations. (* p < 0.05). (D) Relative changes in abundance of microbiota species (Clostridium leptum, Bacteroides caccae, Bifidobacterium pseudolongum) at the baseline and after HY7302 treatment.
Supplier Page from Abcam for Mouse IL-20 ELISA Kit