Fig 1: Silencing of CLDN1 and EGFR in PTC cells enhances co-culture activity and cytotoxicity of CD8+ T cells. A MTT assay measured the activity of co-cultured CD8+ T cells; B ELISA assessed the level of GZMB in the cell culture medium; C ELISA determined the level of IFN-γ in the cell culture medium. *Indicates P < 0.05 compared to the sh-NC group, with experiments repeated three times
Fig 2: IFNκ increased the efficiency of CAR T cells in vitro. A, Analysis of cytotoxicity of CAR T cells against H460 and H322 cells at the indicated E:T ratios for 16 hours. B, CD69 expression in CAR T cells was analyzed after incubation with H460 cells for 16 hours. C, Release of perforin and GzmB from the supernatant was determined using ELISA after incubation with H460 cells for 16 hours. D, PD1 and TIM-3 expression in CAR T cells was examined after incubation with H460 cells. E, eFlour 670 was analyzed in CAR T cells by incubating the cells with H460 cells for 0 or 7 days, respectively. F, CAR T-cell differentiation was analyzed by incubation with H460 cells. Each value represents the mean ± SD, n = 3. *, P < 0.05; **, P < 0.01; ***, P < 0.001. The experiments were independently performed three times, and the results from one representative experiment are displayed. Student t test, and one- and two-way ANOVA were used. E:T, effector to target; GzmB, granzyme B; MFI, mean fluorescence intensity.
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