Fig 1: Study flow chart showing the inclusion of COVID-19 survivors 6 months after discharge. COVID-19, coronavirus disease 2019; CT, computed tomography; ILD, interstitial lung disease; RAGE, receptor for advanced glycation end products; SP-D, surfactant protein D; vWF, von Willebrand factor.
Fig 2: NCI-H441 (A–D) and EA.hy926 (E–H) cell lines characterization using expression of specific proteins. (A) Shows the ΔCq of various mRNA targets used to characterize NCI-H441 (Occludin, ZO-1, SP-A and SP-D) compared with housekeeping genes. (B) Shows Western blotting band density on NCI-H441 cells, of three proteins of interest: Occludin, SP-D and ZO-1. (C,D) shows NCI-H441 cells in which respectively ZO-1 and Occludin are labelled with Alexa-647® (red). (E) Shows the ΔCq of the mRNA targets used to characterize EA.hy926 (vWF and eNOS) compared with housekeeping genes. (F) Shows Western blotting band density of three proteins of interest: eNOS, vWF and CD31. (G,H) show EA.hy926 cells in which vWF, an endothelial protein, is labelled with Alexa488® (green) and eNOS, an enzyme expressed by the vascular endothelium, is labelled with Alexa647® (red), respectively. All images were captured with a confocal microscope, nuclei were labelled with DAPI (blue) and scale bars are 20 µm. Histograms results are mean from 3 separate experiments of triplicates ±SEM. Western blot histogram normalizes the signal with stain-free technology (total proteins).
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