DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Stroma-oriented tumor microenvironment facilitates intraosseous ameloblastoma tumor growthiScienceSeptember 18, 2026Da-Yo Yuh, Chi-Ju Yao, Chia-Yu Chen, Ching-Yi Wu, Jian-Liang Chou, Hui-Na Chou, Ling-Yu Kung, Yi-Tzu Chen, Wei-Chin Chang, Yuan-Wu Chen, et al.room temperature in the dark. Finally, nuclei were counterstained with DAPI Staining Solution (Abcam, #ab228549) and images were acquired using the THUNDER Imager Live Cell (Leica) and processed with Imaris Viewer. Sphere

(2) Suidae iPSC-derived macrophages as models for investigating susceptibility and resilience to African swine fever virusStem Cell ReportsSeptember 8, 2026Tom M. Watson, Lynnette C. Goatley, Stephen Meek, Lel Eory, Sylvia Kohler, Nicholas Berkley, Sofia Sternberg, Melany Jackson, Amy Findlay, Isabella Hoskins, et al.were dried overnight, stained with KaryoMAX Giemsa stain (Thermo Fisher: #10092013) and DAPI (Abcam: ab228549). 20 metaphase spreads were assessed per cell line by light microscopy and chromosomes were counted

(3) Acid-Responsive Zeolitic Imidazolate Framework-8 Core-Shell Nanovesicles Loaded with Interleukin-2 Nanoplatforms Selectively Expand Regulatory T Cells to Reconstruct Immune-Metabolic Homeostasis in Diabetic NeuropathyBiomaterials ResearchSeptember 4, 2026Yangjie Li, Yanqing Liu, Fei Gong, Kangling Xie, Ling Zhang, Taolin Fan, Cui Li, Jiahao Li, Fan Hu, Ying Caiparaformaldehyde (Biosharp, BL539A) for 10 min and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Abcam, ab228549). Confocal images were acquired using a Leica TCS SP8 microscope (Leica Microsystems). All imaging parameters

(4) FGF18 aggravates angiotensin II-induced vascular smooth muscle cell dysfunction via the FGFR-ERK/AKT-GSK3β/β-catenin pathwayJournal of Thoracic DiseaseAugust 31, 2026Yunjun He, Tianchi Chen, Yilang Xiang, Donglin Li, Hongkun Zhang, Ziheng Wu, Yuan Heand left overnight at 4 ℃. Following a wash with PBS, 4’,6-diamidino-2-phenylindole (DAPI) (cat. no. ab228549; Abcam) staining was used to visualize the nuclei after Alexa Fluor 488-labeled secondary antibodies

(5) Combining Napsin A expression with circulating tumor cell counts for enhanced identification of pulmonary adenocarcinoma in ground-glass nodulesTranslational Cancer ResearchAugust 31, 2026Suhong Ruan, Hongjie Yu, Zhenyuan Song, Xiang Zhu, Guangda Yuan, Hongya Xie, Chuanyu Zhang, Tengteng WeiPBS. Nuclei were labeled using 4, 6-Diamino-2-phenylindole (DAPI) staining solution (Abcam, USA, Cat# ab228549). The treated dishes were scanned by the high-throughput capture Cytell Cell Imaging System (GE Healthcare

(6) PVT inhibition mitigates neuropathology and cognitive deficits in chronic cerebral hypoperfusioniScienceAugust 21, 2026Yuying Guan, Jinghao Ma, Rui Li, Jialu Xu, Taoyue Xu, Gaifen Li, Le Sunin the dark with Alexa Fluor 647-conjugated secondary antibody (ab150079, Abcam, 1:1000) and DAPI (ab228549, Abcam, 1:1000) for 20 min. All steps were performed under light-protected conditions to preserve fluorescence,

(7) Metabolic atlas of early human cortex reveals glycolytic remodeling and pentose phosphate pathway control of cell fate transitionsCellAugust 4, 2026Jessenya Mil, Jose A. Soto, Abigail S. Krall, Julia Peloggia, Sara Frigui, Olivia S. Fong, Laila Sathe, Nedas Matulionis, Francesca Day, Linsey Stiles, et al.X100-100ML "MACS® BSA Stock Solution, Bovine serum albumin (BSA)",Miltenyi,Cat# 130-091-376 DAPI,Abcam,Cat# ab228549 ProLong Gold Antifade Mountant,Thermo Fisher Scientific/Invitrogen,Cat# P36934 "ReadyProbes Mouse-on-Mouse

(8) An Efficient Method for Neuron-Like Differentiation of SH-SY5Y Neuroblastoma Cells Using Retinoic Acid and Laminin-Rich Extracellular MatrixGenesisAugust 2, 2026Marina Mantellatto Grigoli, Bianca Cruz Pachane, Angelina Maria Fuzer, Sabrina Dorta de Oliveira, Ana Beatriz Aparecida Targas, Vanessa Alexandre-Silva, Heloisa Sobreiro Selistre-de-Araujo, Patricia Regina Manzine, Marcia Regina Cominetticounterstained with phalloidin‐A488 (Molecular Probes Cat# A12379) and DAPI (0.5 mg/mL, Abcam Cat# ab228549) before slide assembly using the Fluoromount G mounting media (EM Sciences Cat# 17984‐25). Cells were

(9) Embryo vitrification disturbs the pre-implantation DNA demethylation process and affects hepatic functions to threaten long-term metabolic health in miceJournal of Advanced ResearchAugust 1, 2026Yue Ying, Yang Yang, Xilin Shen, Yuli Qian, Jianpeng Chen, Yanyun Ying, Hao Jin, Yazeed Allan, Yifeng Lin, Feixia Wang, et al.% BSA in PBS and incubated with secondary antibodies for 1 h at room temperature followed by DAPI (#ab228549, Abcam) staining. The primary antibodies were used as follows: Rabbit monoclonal anti-TET2 (1:400; #36449,

(10) Transcriptomic Study of Mechanism Driving Conjunctival Fibrosis Following Glaucoma Filtration SurgeryBiomedicinesJuly 31, 2026Zoe Pasvanis, Antony Boynes, Roy C. K. Kong, Elsa C. Chan, Raymond C. B. Wong, Jennifer Fan Gaskincounterstaining was conducted with the application of 4′,6-diamidino-2-phenylindole (DAPI) (1:1000; ab228549; Abcam, Cambridge, UK) for 10 min in the dark at room temperature. Finally, coverslips were mounted

(11) Focal astrocyte loss reveals nuclear translocation during lesion repopulationNat NeurosciJuly 23, 2026Marina Herwerth, Matthias T. Wyss, Nicola B. Schmid, Anna Lasne, Jacqueline Condrau, Luca Ravotto, José María Mateos Melero, Andres Kaech, Gustav Bredell, Carolina Thomas, et al.stainings, slices were incubated with the appropriate secondary antibodies and DAPI (1:1,000; Abcam, ab228549) for nuclear counterstaining. Confocal microscopy Samples were scanned with Zeiss confocal microscopes

(12) Connexin 50 mediates disease-relevant alpha-synuclein oligomer propagation and neuroinflammation in neurodegenerative diseaseiScienceJuly 17, 2026Nicha Puangmalai, Urmi Sengupta, Nemil Bhatt, Nopparat Suthprasertporn, Fadhl Al-Shaebi, Nikita Shchankin, Jia Yi Liew, Twisha Chatterjee, Dhvani Pansuriya, Ahmet E. Balci, et al.receptor blocker (BD Bioscience, 553141). For cell population gating, DAPI staining solution (Abcam, ab228549) for live-dead cell gating and immunolabeling with APC-labeled ACSA-2 antibody (Miltenyi Biotec, 130-117-535)

(13) Mitochondrial-generated G-quadruplex-forming lncRNAs regulate heme homeostasisiScienceJuly 17, 2026Vinodh J. Sahayasheela, Ryohei Noizumi, Manendra B. Lankadasari, Takumi Terada, Mitsuharu Ooga, Atharv Kulkarni, Shinjiro Suzuki, Takuya Hidaka, Li Cai, Ganesh N. Pandian, et al.,Thermo Fisher Scientific,Cat# M22426 Hoechst-33342,Thermo Fisher Scientific,Cat# H3570 DAPI Staining Solution,Abcam,Cat# ab228549 ProLong Gold Antifade Mountant,Thermo Fisher Scientific,Cat# P36930 PolyJet transfection reagent,SignaGen,Cat#

(14) A peptide-based screen for cell death inhibitors identifies the cytoprotective compound CDL36bioRxivJuly 10, 2026Zintis Inde, Sebastian Keppler, Jesse Gelles, Cameron S. Fraser, Adam Presser, Jarvier N. Mohammed, May Jung, David S. Garvey, Tudor Moldoveanu, Jerry E. Chipuk, et al.cytochrome c antibody (Biolegend, cat #612310) diluted 1:1000 and DAPI staining solution (Abcam, cat #ab228549) diluted 1:100. 10 μL/well staining solution was added and incubated to stain for 24 hours at 4 °C with

(15) Compound 4′-O-methylbroussochalcone B attenuates LPS/ATP-induced macrophage pyroptosis in vitro through modulation of the NLRP3 inflammasome and MAPK/NF-κB pathwaysFrontiers in PharmacologyJune 24, 2026Jihee Choi, You Chul Chung, Ami Lee, Han Na Kang, Youn-Hwan Hwangwere incubated with propidium iodide (PI; P3566) for 5 min and counterstained with Hoechst 33,258 (ab228549, Abcam). Fluorescence images were captured using an ImageXpress Micro 4 High-Content Screening System

(16) Targeting the 4EBP1/HSP90β/Nrf2 Axis Sensitizes β-catenin-mutant Hepatocellular Carcinoma to mTOR Inhibitors via Ferroptosis InductionJ Clin Transl HepatolJune 16, 2026Rong Li, Yi Zhou, Zimu Wang, Gang Liu, Deyu Fan, Lanxuan Huang, Fule Deng, Ning Wei, Runze Shang, Meng Xufluorescence-labeled secondary antibodies at room temperature for 2 h. Nuclei were stained with DAPI (ab228549, Abcam). Finally, images were captured using an LSM880 confocal microscope (Leica, Germany). GSH/GSSG

(17) Hypoxia‐preconditioned adipose‐derived mesenchymal stem cells‐derived exosomes transferring H19 obstruct neutrophil extracellular traps formation via HOXA5 ‐mediated inactivation of TLR4 / NF ‐ κB / NLRP3 inflammatory signalingJournal of Diabetes InvestigationJune 8, 2026Li Qian, Xianxi Meng, Bairong Fang, Li PiAbcam) for 2 h and then incubated with the 4′,6‐diamidino‐2‐phenylindole (DAPI) staining solution (#ab228549, Abcam) for 5 min. Fluorescence images (at ×400 magnification) were observed under a SP8 LIGHTNING confocal

(18) A Human Vocal Fold Organ-On-Chip for Studying Platform-Dependent Mucosal Responses to Particulate MatterbioRxivJune 2, 2026Patrick T. Coburn, Meghana Munipalle, Yin Liu, Vlasta Lungova, Samjhana Thapa, Lisa Martignetti, Xinyu Liu, Gilles Maussion, Carol X.-Q. Chen, Thomas M. Durcan, et al.anti-chicken (1:1000, ThermoFisher A-21103)",Intermediate filaments DAPI,Nuclear DNA,—,1:5000,Abcam ab228549,—,—,Counter-staining TaqMan assays for quantitative real-time PCR,TaqMan assays for quantitative real-time PCR

(19) Neuregulin-1 promotes early regenerative and autophagic responses after ischemic stroke via spatial proteomicsFront Cell NeurosciJune 2, 2026Jessica M. Noll, Christopher McGinley, Catherine J. Augello, Esra Kürüm, Liuliu Pan, Anna Pavenko, Andy Nam, Byron D. Ford, Gregory D. Fordfor 2 min. Sections were then incubated in a freshly prepared solution of 0.0004% FJB with DAPI (Cat# ab228549, Abcam, Waltham, MA) for 20 min, rinsed in distilled water 3 times for 2 min, and then dried at 50°C. For

(20) Herkinorin Exerted Neuroprotective Effects After Spinal Cord Injury by Suppression of NF-κB Pathway ActivationNeurochem ResJune 1, 2026Jianyong Wang, Jiacheng Yu, Wen Han, Chaochao Ding, HaiFeng Wang, Siqi Chen, Yi Huang, Wenting Lan(Goat anti-Rabbit IgG H&L (Alexa Fluor®488), Abcam, ab150077; Goat anti-Mouse IgG H&L (Alexa Fluor®488), Abcam, ab150113) and DAPI (Abcam, ab228549). Images were acquired using a fluorescence microscope (TCS SP8, Lecia) Six random fields from three

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(1)

6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

Abcam

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DAPI Staining Solution from Abcam

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