DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(21) Gypenoside XLIX ameliorates diabetic retinopathy by downregulating prostaglandin-endoperoxide synthase 2 in retinal pigment epithelium cells to inhibit ferroptosis and preserve tight junction integrityFrontiers in PharmacologyMarch 23, 2026Jiayi Gu, Manhui Zhu, Lele Li, Xin Cao, Xiaoli Yu, Xiaobo Huang, Lili Huang, Qi Cai, Yan Zhu, Wendie Li, et al.United States). Anti-ZO-1 (1:100; Cat# ab221547) and 4′,6-diamidino-2-phenylindole (DAPI; 1:5,000; Cat# ab228549) from Abcam (Cambridge, United Kingdom). 2.3 Animal grouping and treatment Male C57BL/6 mice (6–8

(22) Low, plasma level‑informed native curcumin concentrations fail to induce cell death in human lung and colorectal cancer cellsPharm BiolMarch 20, 2026Ilma Imtiaz, Janet Schloss, Andrea BugarcicLI-COR Biosciences, while goat anti-rabbit Alexa Fluor® 488 (ab150081) and stains, including DAPI (ab228549) and Rhodamine Phalloidin (ab235138), were purchased from Abcam. Staurosporine (S5921), Triton X-100

(23) Targeting cardiomyocyte-derived extracellular vesicle miR-574-5p protects against cognitive impairment induced by ischemia–reperfusionBMC MedicineMarch 19, 2026Erya Chen, Xiaoyu Zhu, Haiqing Chang, Qi Li, Zhenkun Zhao, Changteng Zhang, Yu Cao, Yajing Wang, Xinliang Ma, Tao Zhu, et al.antibody at room temperature for 1–2 h. Next, the nuclei were stained with DAPI (Abcam, Cambridge, UK, no. ab228549) for 30 min at room temperature. Finally, the tissue sections were covered with coverslips after using

(24) In Vitro Assessment of Ventricular Catheters with a Multilayered Fibrous Web to Prevent Cellular OcclusionACS Biomaterials Science & EngineeringMarch 9, 2026Seunghyun Lee, Amirhossein Shahriari, Gio Jison, Noah Ramos, Sora Sato, Celine Tran, Leandro Castaneyra-Ruiz, Michael MuhonenA11034) was applied for 1 h. Finally, cells were stained with DAPI (4′,6-diamidino-2-phenylindole, ab228549, Abcam) (1:5000 in PBS) for nuclear labeling. ChPE cell cultures were labeled with antitransthyretin

(25) Age-mimicking hydrogel stiffness recapitulates the mechanical niche of the hippocampus to regulate neural stem cell senescenceMater Today BioMarch 3, 2026Luyao Guo, Longjiao Ge, Yong Li, Shouye Wang, Huitong Li, Xiaoyu Wang, Weiliang Qian, Yu Zhang, Liuhanhui Guo, Luxuan Guo, et al.(Jackson, 711-165-152, 1:1000), donkey anti-rat Cy3 (Jackson, 712-165-153, 1:1000) and DAPI (Abcam, ab228549, 1:1000). After removing the secondary antibodies and washing the sections three times with PBS, the

(26) Retrotransposon activation during spermatogenesis achieves massive ecDNA biogenesis, but rare integrationGenes & DevelopmentMarch 2, 2026Lauren Tracy, Yao Chen, Zz Zhao Zhangroom temperature. Then samples were washed three more times in PBST, with DAPI (1:1000, Abcam Cat. # ab228549) in the second wash step. Finally, the samples were mounted with Vectashield Mounting Medium. Images

(27) Extracellular vesicles from stem cells rescue cellular phenotypes and behavioral deficits in SHANK3-associated ASD neuronal and mouse modelsCell Death & DiseaseFebruary 22, 2026Ashwani Choudhary, Idan Rosh, Yara Hussein, Shai Netser, Aviram Shemen, Taghreed Suliman, Wote Amelo Rike, Lilach Simchi, Boris Shklyar, Ahmad Abu-Akel, et al.overnight at 4 °C. The next day, the coverslips were washed in DPBS and incubated with DAPI (Abcam, ab228549, 1:2500) and the corresponding secondary antibodies for 60 min. at room temperature. Then, the coverslips

(28) SNCA triplication disrupts proteostasis and extracellular architecture prior to neurodegeneration in human midbrain organoidsnpj Parkinson's DiseaseFebruary 16, 2026Elpida Statoulla, Maria Zafeiri, Kleanthi Chalkiadaki, Georgia Voudouri, Karmel S. Gkika, Christos Papazoglou, Thomas M. Durcan, Arkady Khoutorsky, Seyed Mehdi Jafarnejad, Sarah Maguire, et al.detailed list of antibodies can be found in Sup. Data 4. DRAQ5 (Abcam, #ab108410) or DAPI (Abcam, #ab228549) were used for nuclear staining. Imaging was performed using a LEICA SP5 and a NIKON A1R HD confocal

(29) Cell jamming transition is regulated by mitochondrial pyruvate transport and endocytosisbioRxivFebruary 13, 2026Alexandra Bermudez, Zoe Latham, Johnny Diaz, Weihong Yan, Jerry Chen, Dapeng Bi, Andrew Goldstein, Jimmy K Hu, Neil Y.C. Linanti-rabbit Alexa Fluor 647 (Invitrogen, A21042 and 4414S, respectively)—together with DAPI (Abcam, ab228549) and either phalloidin (Invitrogen, A12379) or actin stain (Invitrogen, R37110). Microscopy and Live-cell Imaging

(30) Obesity Reprogrammes Adipose Extracellular Vesicles to Induce Muscle Atrophy via miR-150-5p-Mediated Transcriptional SilencingJ Cachexia Sarcopenia MuscleFebruary 3, 2026Joshua M. J. Price, Michael Macleod, Thomas Nicholson, Caitlin M. Ditchfield, Bethy Airstone, Natalie Lachlan-Jiraskova, Edward T. Davis, Kostas Tsintzas, Simon W. Jonesat RT. Wells were then washed with PBS, after which DAPI staining solution (1:5000 in PBS, Abcam, #ab228549) was added for 5 min in the dark. Finally, wells were washed again with PBS, before Fluoromount Aqueous Mounting Medium

(31) Astrocytogenic bidirectional plasticity at spinal nociceptive synapses regulates acute nociceptive processingPainFebruary 1, 2026Sibel Ada, Laura Klinger, Hannah L. Teuchmann, Valeria Mussetto, Viktoria Hadschieff, Mira T. Kronschläger, Anna S.M. Siegert, Lidia Trofimova, Raphael Holzinger, Danijela Kurija, et al.×1 PBS (3 × 10 minutes) and incubated in ×1 PBS + 0.2% 40,6-diamidino-2-phenylindole (DAPI, Abcam, ab228549), protected from light, for 60 minutes. Slices were again washed with ×1 PBS (2 × 5 minutes) and mounted

(32) AX-2: A Promising Non-Hemolytic Protein of Bacillus thuringiensis with Potent Selective Cytotoxicity Against Breast Cancer CellsMoleculesJanuary 29, 2026Alain Cruz-Nolasco, Miguel Angel Peña-Rico, Sibel J. Estrada-Escobedo, Angel A. Ortela-Gregorio, Erick A. Juarez-Arellano, Genaro Vázquez-Victorio, Angelica S. Martinez-Ramirez, Michele Rorato Sagrillo, Roberto C. Vianna Santos, Luz Camacho, et al.RT. Excess liquid was removed by aspirating around the sample. Next, 1 µg/mL DAPI staining solution (ab228549, Abcam, Cambridge, UK) was added and incubated for 5 min. Antifade mounting media was added and coverslips

(33) VEGF-A isoforms induce the expression of APLN in endothelial cells during human prenatal lung developmentFrontiers in Cell and Developmental BiologyJanuary 23, 2026Antony Hoarau, Andrew Frauenpreis, Randa Belgacemi, Emma Loeffler, Osshaya Maalouf, Ian A. Glass, Denise Al Alam, Soula Danopoulos,Thermo Fisher,MA5-13188,1/100 Claudin-5,LSBio,LS-C352946,1/200 Ki67,Epredia,RM-9106-S1,1/200 DAPI,Abcam,AB228549,1/1000 RNAscope assay,RNAscope assay,RNAscope assay,RNAscope assay APLN-C3,ACDB,44–9971-C3,1/50 Secondary

(34) Optimal murine CD4+ T cell priming by mRNA-lipid nanoparticle vaccines requires endogenous antigen processingNature CommunicationsJanuary 16, 2026Julia E. Rood, Suh Kyung Yoon, Mary K. Heard, Stephen D. Carro, Emma J. Hedgepeth, Mary E. O’Mara, Michael J. Hogan, Nhu Le, Hiromi Muramatsu, Kieu Lam, et al.(Invitrogen A21131, diluted to 10 μg/mL) to boost NP signal, followed by staining with DAPI (Abcam AB228549, diluted 1:1000) for 5 minutes. Muscle sections were additionally stained with wheat germ agglutinin

(35) 3D immunolabelling of human aortic valves by optimized CUBIC tissue clearing and its application for neural mapping in aortic stenosisEuropean Heart Journal OpenJanuary 6, 2026Justine Bergeon, David Hupin, Jianjiang Hu, Sylvie Le Guyader, Anders Franco-Cereceda, Magnus Bäckconjugated to Alexa Fluor 647. Nuclear staining was performed using DAPI (1:15 000 dilution, Abcam, ab228549). α7nAchR were labelled with first with the primary antibody, anti-nicotinic acetylcholine receptor alpha 7

(36) Permeability-driven pressure and cell proliferation control lumen morphogenesis in pancreatic organoidsNature Cell BiologyJanuary 1, 2026Byung Ho Lee, Kana Fuji, Heike Petzold, Phil Seymour, Siham Yennek, Coline Schewin, Allison Lewis, Daniel Riveline, Tetsuya Hiraiwa, Masaki Sano, et al.4 °C. To stain nuclei, Hoechst solution (34580, Invitrogen) or 4,6-diamidino-2-phenylindole (DAPI; ab228549, Abcam) was added in 0.25% Triton, 1% BSA in PBS for 4 h at room temperature after the incubation with

(37) Multifunctional copper-based metal-organic frameworks/chitosan derivative/exosome composite sponge for skin healingiScienceDecember 19, 2025Heng Zhou, Jiaqi Wang, Yu Ding, Huan Liang, Tao Qin, Pengjiao Wang, Xiuli Gao, Xiaoqing HeLtd.",Cat# 01000433 BTC,"Titan Scientific Co., Ltd.",Cat# 011801124 DAPI,"Abcam Trading Co., Ltd.",Cat# ab228549 CS,"Shanghai Ron Biotechnology Co., Ltd.",Cat# R051510 TMG,"Titan Scientific Co., Ltd.",Cat# 01089722 EDC,"Titan

(38) Positive modulation of sigma-1 receptor: a new weapon to mitigate disease progression in amyotrophic lateral sclerosisTransl NeurodegenerDecember 15, 2025Julien Le Friec, Hugo Mourier, Simon Couly, Nicolas Cubedo, Kevin Dubois, Johann Meunier, Benjamin Delprat, Arnaud De Zordo-Banliat, Tahar Ayad, David Virieux, et al.Finally, sections were briefly washed with 1× TBS, incubated for 4 min with DAPI (dilution 1:30,000; ab228549; Abcam, Cambridge, UK) and washed in water. Slides were mounted using FluoroSaveTM Reagent (345789;

(39) Heat-shock pathway activation by TRC051384 protects spiral ganglion neurons from noise-induced hearing lossbioRxivDecember 11, 2025Jintao Yu, Miguel A. Ramirez, Yi-Zhi Wang, Seby Edassery, Maxwell Shramuk, Mary Ann Cheatham, Mark A. Rutherford, Leah J Welty, Jeffrey N Savasincubation, samples were washed three times in PBS (15 min each) and counterstained with DAPI solution (Abcam ab228549) for 5 min. Tissues were mounted using ProLong Gold Antifade Mountant (Thermo Scientific, Cat# P10144).

(40) MEF2C controls segment-specific gene regulatory networks that direct heart tube morphogenesisGenes & DevelopmentDecember 1, 2025Jonathon M. Muncie-Vasic, Tanvi Sinha, Alexander P. Clark, Emily F. Brower, Jeffrey J. Saucerman, Brian L. Black, Benoit G. Bruneauthe same blocking buffer with gentle rotation at 37°C. Secondary antibodies and 1 µg/mL DAPI (Abcam ab228549) were added in a blocking buffer consisting of PBS (Life Technologies 14190250), 5% normal donkey serum

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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